RGD Reference Report - Identification by sequence analysis of a second rat brain cDNA encoding the dopamine (D2) receptor. - Rat Genome Database

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Identification by sequence analysis of a second rat brain cDNA encoding the dopamine (D2) receptor.

Authors: Miller, JC  Wang, Y  Filer, D 
Citation: Miller JC, etal., Biochem Biophys Res Commun 1990 Jan 15;166(1):109-12.
RGD ID: 728513
Pubmed: PMID:2137336   (View Abstract at PubMed)

A rat brain cDNA library constructed in lambda ZAP II was screened with three oligonucleotide probes based on the reported coding region of the D2 receptor gene, RGB-2. A complete cDNA clone, D2(8)-1, showing positive signals with the three probes was subsequently identified by restriction analysis and dideoxy sequence analysis to be a variant of the RGB-2 gene. Comparison of the two genes revealed almost complete homology except that D2(8)-1 contains an 87 bp insert within the protein coding region and 265 additional nucleotides 5' upstream from the 5' end reported for RGB-2. It is suggested that at least two mRNA species encoding for D2 receptors exist in rat brain, possibly resulting from alternative splicing of RNA.

Objects referenced in this article
Gene Drd2 dopamine receptor D2 Rattus norvegicus

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