RGD Reference Report - GABAA receptor beta subunit heterogeneity: functional expression of cloned cDNAs. - Rat Genome Database

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GABAA receptor beta subunit heterogeneity: functional expression of cloned cDNAs.

Authors: Ymer, S  Schofield, PR  Draguhn, A  Werner, P  Kohler, M  Seeburg, PH 
Citation: Ymer S, etal., EMBO J 1989 Jun;8(6):1665-70.
RGD ID: 728451
Pubmed: PMID:2548852   (View Abstract at PubMed)
PMCID: PMC401007   (View Article at PubMed Central)

Cloned cDNAs encoding two new beta subunits of the rat and bovine GABAA receptor have been isolated using a degenerate oligonucleotide probe based on a highly conserved peptide sequence in the second transmembrane domain of GABAA receptor subunits. The beta 2 and beta 3 subunits share approximately 72% sequence identity with the previously characterized beta 1 polypeptide. Northern analysis showed that both beta 2 and beta 3 mRNAs are more abundant in the brain than beta 1 mRNA. All three beta subunit encoding cDNAs were also identified in a library constructed from adrenal medulla RNA. Each beta subunit, when co-expressed in Xenopus oocytes with an alpha subunit, forms functional GABAA receptors. These results, together with the known alpha subunit heterogeneity, suggest that a variety of related but functionally distinct GABAA receptor subtypes are generated by different subunit combinations.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Gabrb1RatGABA-A receptor activity  IDA  RGD 
Gabrb3RatGABA-A receptor activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gabrb1  (gamma-aminobutyric acid type A receptor subunit beta1)
Gabrb3  (gamma-aminobutyric acid type A receptor subunit beta 3)

Objects referenced in this article
Gene Gabrb2 gamma-aminobutyric acid type A receptor subunit beta 2 Rattus norvegicus

Additional Information