RGD Reference Report - Cloning of AL-1, a ligand for an Eph-related tyrosine kinase receptor involved in axon bundle formation. - Rat Genome Database

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Cloning of AL-1, a ligand for an Eph-related tyrosine kinase receptor involved in axon bundle formation.

Authors: Winslow, JW  Moran, P  Valverde, J  Shih, A  Yuan, JQ  Wong, SC  Tsai, SP  Goddard, A  Henzel, WJ  Hefti, F 
Citation: Winslow JW, etal., Neuron 1995 May;14(5):973-81.
RGD ID: 728210
Pubmed: PMID:7748564   (View Abstract at PubMed)

REK7 is an Eph-related tyrosine kinase receptor expressed exclusively in the nervous system, predominantly in hippocampus and cortex. A soluble REK7-IgG fusion protein, produced to analyze the biological role of REK7, prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation. Using REK7-IgG as an affinity reagent, we purified and cloned a novel REK7 ligand called AL-1, a GPI-linked protein homologous to other members of an emerging ligand family. Membrane attachment of AL-1 appears necessary for receptor activation, since REK7 on cortical neurons is efficiently activated by transfected cells expressing GPI-linked AL-1, but not by soluble AL-1. Consistent with this, soluble AL-1 blocks axon bundling. Our findings, together with the observation that both molecules are expressed in the brain, suggest a role in the formation of neuronal pathways, a crucial feature of nervous system development and regeneration.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
axonal fasciculation  NAS 728210 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
axon located_inIDA 728210PMID:7748564UniProt 
plasma membrane located_inIDA 728210PMID:7748564UniProt 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
ephrin receptor binding  IDA 728210 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Efna5  (ephrin A5)
Epha5  (EPH receptor A5)


Additional Information