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Cloning of the Dck gene encoding rat deoxycytidine kinase.

Authors: Stegmann, AP  Honders, MW  Willemze, R  Landegent, JE 
Citation: Stegmann AP, etal., Gene 1994 Dec 15;150(2):351-4.
Pubmed: (View Article at PubMed) PMID:7821805

In order to study the mutational inactivation of deoxycytidine kinase (Dck) in a rat model for acute myeloid leukemia we have cloned the complete coding region of the rat Dck gene. Using primers chosen from the human Dck cDNA sequence, we obtained a rat-specific probe via PCR and used it to isolate two clones from a rat lymphocyte cDNA library. The ORF showed 89.7 and 92.2% nucleotide identity with the human and mouse Dck, respectively, and encodes a 260-amino-acid protein, that is 91.9 and 94.6% homologous to human and mouse Dck, respectively. Northern blot analysis of rat tissues revealed high expression of a 4.1-kb Dck transcript in the thymus, whereas spleen, liver and lung samples showed weak expression of the gene. This tissue-specific expression pattern was confirmed by cDNA-PCR analysis.

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RGD ID: 727775
Created: 2003-11-05
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Last Modified: 2004-05-25
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