RGD Reference Report - Molecular cloning and expression of cDNAS encoding rat aldosterone synthase: variants of cytochrome P-450(11 beta). - Rat Genome Database

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Molecular cloning and expression of cDNAS encoding rat aldosterone synthase: variants of cytochrome P-450(11 beta).

Authors: Matsukawa, N  Nonaka, Y  Ying, Z  Higaki, J  Ogihara, T  Okamoto, M 
Citation: Matsukawa N, etal., Biochem Biophys Res Commun 1990 May 31;169(1):245-52.
RGD ID: 727645
Pubmed: PMID:2350348   (View Abstract at PubMed)

Two distinct forms of cDNA encoding rat aldosterone synthase were cloned from an adrenal capsular tissue cDNA library. The deduced amino acid sequences showed that one of the enzymes (P-450(11 beta),aldo-1) had a long extension peptide composed of 34 amino acid residues while the other (P-450(11 beta),aldo-2) had an extension peptide identical to that of rat P-450(11 beta). Glu at the 320th position of P-450(11 beta),aldo-1 was replaced with Lys in P-450(11 beta),aldo-2. The amino acid sequence of the aldosterone synthase was highly homologous (81%) to rat P-450(11 beta). Constructed expression vector containing the cDNA for extension peptide of P-450(11 beta) and the mature protein of P-450(11 beta),aldo-1 was transfected into COS-7 cells. The cells converted 11-deoxycorticosterone into corticosterone, 18-hydroxycorticosterone, and aldosterone.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
corticosterone 18-monooxygenase activity  IDA 727645 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cyp11b2  (cytochrome P450, family 11, subfamily b, polypeptide 2)

Objects referenced in this article
Gene Cyp11b1 cytochrome P450, family 11, subfamily b, polypeptide 1 Rattus norvegicus

Additional Information