RGD Reference Report - Primary structure and functional expression of a developmentally regulated skeletal muscle chloride channel. - Rat Genome Database

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Primary structure and functional expression of a developmentally regulated skeletal muscle chloride channel.

Authors: Steinmeyer, K  Ortland, C  Jentsch, TJ 
Citation: Steinmeyer K, etal., Nature 1991 Nov 28;354(6351):301-4.
RGD ID: 727583
Pubmed: PMID:1659664   (View Abstract at PubMed)
DOI: DOI:10.1038/354301a0   (Journal Full-text)

Skeletal muscle is unusual in that 70-85% of resting membrane conductance is carried by chloride ions. This conductance is essential for membrane-potential stability, as its block by 9-anthracene-carboxylic acid and other drugs causes myotonia. Fish electric organs are developmentally derived from skeletal muscle, suggesting that mammalian muscle may express a homologue of the Torpedo mamorata electroplax chloride channel. We have now cloned the complementary DNA encoding a rat skeletal muscle chloride channel by homology screening to the Cl- channel from Torpedo. It encodes a 994-amino-acid protein which is about 54% identical to the Torpedo channel and is predominantly expressed in skeletal muscle. Messenger RNA amounts in that tissue increase steeply in the first 3-4 weeks after birth, in parallel with the increase in muscle Cl- conductance. Expression from cRNA in Xenopus oocytes leads to 9-anthracene-carboxylic acid-sensitive currents with time and voltage dependence typical for macroscopic muscle Cl- conductance. This and the functional destruction of this channel in mouse myotonia suggests that we have cloned the major skeletal muscle chloride channel.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Clcn1Ratchloride transport  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Clcn1Ratvoltage-gated chloride channel activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Clcn1  (chloride voltage-gated channel 1)

Objects referenced in this article
Gene Clcn1 chloride channel, voltage-sensitive 1 Mus musculus

Additional Information