RGD Reference Report - Pleiotrophin/heparin-binding growth-associated molecule as a mitogen of rat hepatocytes and its role in regeneration and development of liver. - Rat Genome Database

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Pleiotrophin/heparin-binding growth-associated molecule as a mitogen of rat hepatocytes and its role in regeneration and development of liver.

Authors: Asahina, K  Sato, H  Yamasaki, C  Kataoka, M  Shiokawa, M  Katayama, S  Tateno, C  Yoshizato, K 
Citation: Asahina K, etal., Am J Pathol 2002 Jun;160(6):2191-205.
RGD ID: 727382
Pubmed: PMID:12057922   (View Abstract at PubMed)
PMCID: PMC1850835   (View Article at PubMed Central)
DOI: DOI:10.1016/S0002-9440(10)61167-4   (Journal Full-text)

Previously pleiotrophin (PTN) was identified among proteins secreted by Swiss 3T3 cells as a mitogen for cultured adult rat hepatocytes. The present study showed that the growth of rat hepatocytes was enhanced when cultured with rat hepatic stellate cells (HSCs). HSCs expressed PTN mRNA and secreted its protein in the co-cultures. Recombinant PTN enhanced the growth of hepatocytes in culture, suggesting that HSCs stimulate the growth of hepatocytes through the action of PTN. To know the biological role of PTN in the growth of hepatocytes in vivo, we examined the expression of PTN in four regeneration models of adult liver and embryonic liver of rat. The expression of PTN mRNA in the liver was markedly up-regulated by the treatment with D-galactosamine (GalN) or with acetylaminofluorene followed by partial hepatectomy. HSCs expressed PTN mRNA in response to GalN treatment and its protein was found on hepatocytes. The mRNA expression of N-syndecan, a PTN receptor, was up-regulated in GalN-treated hepatocytes. The mesenchymal cells in the septum transversum enclosing the embryonic liver, but not embryonic HSCs, expressed PTN mRNA. We suggest that PTN is secreted from activated adult HSCs and embryonic mesenchymal cells as a mitogen of parenchymal cells in adult and embryonic liver, respectively.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to organic cyclic compound  IEP 727382D-galactosamineRGD 
liver development  IEP 727382 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
extracellular region  IDA 727382 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ptn  (pleiotrophin)

Objects referenced in this article
Gene Sdc3 syndecan 3 Rattus norvegicus

Additional Information