RGD Reference Report - Identification of Ca2+-dependent binding partners for the neuronal calcium sensor protein neurocalcin delta: interaction with actin, clathrin and tubulin. - Rat Genome Database

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Identification of Ca2+-dependent binding partners for the neuronal calcium sensor protein neurocalcin delta: interaction with actin, clathrin and tubulin.

Authors: Ivings, L  Pennington, SR  Jenkins, R  Weiss, JL  Burgoyne, RD 
Citation: Ivings L, etal., Biochem J 2002 May 1;363(Pt 3):599-608.
RGD ID: 727364
Pubmed: PMID:11964161   (View Abstract at PubMed)
PMCID: PMC1222513   (View Article at PubMed Central)

The neuronal calcium sensors are a family of EF-hand-containing Ca(2+)-binding proteins expressed predominantly in retinal photoreceptors and neurons. One of the family members is neurocalcin delta, the function of which is unknown. As an approach to elucidating the protein interactions made by neurocalcin delta, we have identified brain cytosolic proteins that bind to neurocalcin delta in a Ca(2+)-dependent manner. We used immobilized recombinant myristoylated neurocalcin delta combined with protein identification using MS. We demonstrate a specific interaction with clathrin heavy chain, alpha- and beta-tubulin, and actin. These interactions were dependent upon myristoylation of neurocalcin delta indicating that the N-terminal myristoyl group may be important for protein-protein interactions in addition to membrane association. Direct binding of neurocalcin delta to clathrin, tubulin and actin was confirmed using an overlay assay. These interactions were also demonstrated for endogenous neurocalcin delta by co-immunoprecipitation from rat brain cytosol. When expressed in HeLa cells, neurocalcin delta was cytosolic at resting Ca(2+) levels but translocated to membranes, including a perinuclear compartment (trans-Golgi network) where it co-localized with clathrin, following Ca(2+) elevation. These data suggest the possibility that neurocalcin delta functions in the control of clathrin-coated vesicle traffic.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
actin binding  IDA 727364 RGD 
alpha-tubulin binding  IDA 727364 RGD 
protein binding  IPINcald (Rattus norvegicus)727364 RGD 
protein binding  IPICltc (Rattus norvegicus)727364 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cltc  (clathrin heavy chain)
Ncald  (neurocalcin delta)

Objects referenced in this article
Gene Gapdh glyceraldehyde-3-phosphate dehydrogenase Rattus norvegicus
Gene Tuba1a tubulin, alpha 1A Rattus norvegicus
Gene Tubb2b tubulin, beta 2B class IIb Rattus norvegicus

Additional Information