RGD Reference Report - Immunophenotypical characterization of macrophages in rat bleomycin-induced scleroderma. - Rat Genome Database

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Immunophenotypical characterization of macrophages in rat bleomycin-induced scleroderma.

Authors: Juniantito, V  Izawa, T  Yuasa, T  Ichikawa, C  Yano, R  Kuwamura, M  Yamate, J 
Citation: Juniantito V, etal., Vet Pathol. 2013 Jan;50(1):76-85. doi: 10.1177/0300985812450718. Epub 2012 Jun 13.
RGD ID: 7257593
Pubmed: PMID:22700848   (View Abstract at PubMed)
DOI: DOI:10.1177/0300985812450718   (Journal Full-text)

Scleroderma is a skin disorder characterized by persistent fibrosis. Macrophage properties influencing cutaneous fibrogenesis remain to be fully elucidated. In this rat (F344 rats) model of scleroderma, at 1, 2, 3, and 4 weeks after initiation of daily subcutaneous injections of bleomycin (BLM; 100 mul of 1 mg/ml daily), skin samples were collected for histological and immunohistochemical evaluations. Immunohistochemically, the numbers of cells reacting to ED1 (anti-CD68; phagocytic activity) and ED2 (anti-CD163; inflammatory factor production) began to increase at week 1, peaked at week 2, and decreased thereafter. In contrast, the increased number of cells reacting to OX6 (anti-MHC class II molecules) was seen from week 2 and remained elevated until week 4. alpha-Smooth muscle actin-positive myofibroblasts were increased for 4 weeks. Double labeling revealed that galectin-3, a regulator of fibrogenic factor TGF-beta1, was expressed in CD68+, CD163+, and MHC class II+ macrophages and myofibroblasts. mRNA expression of TGF-beta1, as well as MCP-1 and CSF-1 (both macrophage function modulators), were significantly elevated at weeks 1 to 4. This study shows that the increased number of macrophages with heterogeneous immunophenotypes, which might be induced by MCP-1 and CSF-1, could participate in the sclerotic lesion formation, presumably through increased fibrogenic factors such as galectin-3 and TGF-beta1; the data may provide useful information to understand the pathogenesis of the human scleroderma condition.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
systemic scleroderma  ISOCsf1 (Rattus norvegicus)7257593; 7257593mRNA:increased expression:skinRGD 
systemic scleroderma  IEP 7257593mRNA:increased expression:skinRGD 

Objects Annotated

Genes (Rattus norvegicus)
Csf1  (colony stimulating factor 1)

Genes (Mus musculus)
Csf1  (colony stimulating factor 1 (macrophage))

Genes (Homo sapiens)
CSF1  (colony stimulating factor 1)


Additional Information