RGD Reference Report - Large dense-core secretory granule biogenesis is under the control of chromogranin A in neuroendocrine cells. - Rat Genome Database

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Large dense-core secretory granule biogenesis is under the control of chromogranin A in neuroendocrine cells.

Authors: Kim, T  Tao-Cheng, JH  Eiden, LE  Loh, YP 
Citation: Kim T, etal., Ann N Y Acad Sci 2002 Oct;971:323-31.
RGD ID: 724711
Pubmed: PMID:12438143   (View Abstract at PubMed)

The large dense-core secretory granule is an organelle in neuroendocrine/endocrine cells, where prohormones and proneuropeptides are stored, processed, and secreted in a regulated manner. Here we present evidence that chromogranin A (CgA), one of the most abundant acidic glycoproteins ubiquitously present in neuroendocrine/endocrine cells, regulates dense-core secretory granule biogenesis. Specific depletion of CgA expression by antisense RNAs in PC12 cells led to a profound loss of secretory granule formation. An exogenously expressed prohormone, pro-opiomelanocortin, was neither stored nor secreted in a regulated manner in CgA-deficient PC12 cells. Overexpression of bovine CgA into CgA-deficient PC12 cells rescued regulated secretion. Other secretory granule proteins, such as chromogranin B (CgB), carboxypeptidase E, and synaptotagmin, were rapidly degraded, whereas nongranule proteins were not affected in CgA-deficient PC12 cells. Unlike CgA, another granin protein CgB could not substitute for the role of CgA in secretory granule biogenesis. Thus, we conclude that CgA is a master "on/off" switch regulating the formation of the dense-core secretory granule in neuroendocrine cells.

Objects referenced in this article
Gene Chga chromogranin A Rattus norvegicus

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