RGD Reference Report - Ornithine decarboxylase antizyme Oaz3 modulates protein phosphatase activity. - Rat Genome Database

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Ornithine decarboxylase antizyme Oaz3 modulates protein phosphatase activity.

Authors: Ruan, Y  Cheng, M  Ou, Y  Oko, R  Van der Hoorn, FA 
Citation: Ruan Y, etal., J Biol Chem. 2011 Aug 19;286(33):29417-27. doi: 10.1074/jbc.M111.274647. Epub 2011 Jun 28.
RGD ID: 7245493
Pubmed: PMID:21712390   (View Abstract at PubMed)
PMCID: PMC3156769   (View Article at PubMed Central)
DOI: DOI:10.1074/jbc.M111.274647   (Journal Full-text)

Ornithine decarboxylase antizyme 3 (Oaz3) is expressed in spermatids, makes up the antizyme family of Oaz genes with Oaz1 and Oaz2, and was proposed to encode a 22 kDa antizyme protein involved in polyamine regulation similar to the 22 kDa OAZ1 and OAZ2 proteins. Here we demonstrate however that the major product encoded by Oaz3 is a 12 kDa protein, p12, which lacks the antizyme domain that interacts with ornithine decarboxylase. We show that p12 does not affect ornithine decarboxylase levels, providing an explanation for the surprising observation made in Oaz3 knock-out male mice, which do not display altered testis polyamine metabolism. This suggested a novel activity for Oaz3 p12. Using immuno-electron microscopy we localized p12 to two structures in the mammalian sperm tail, viz. the outer dense fibers and fibrous sheath, as well as to the connecting piece linking head and tail. We identified myosin phosphatase targeting subunit 3 (MYPT3), a regulator of protein phosphatase PP1beta, as a major p12-interacting protein, and show that MYPT3 is present in sperm tails and that its ankyrin repeat binds p12. We show that MYPT3 can also bind protein phosphatase PP1gamma2, the only protein phosphatase present in sperm tails, and that p12- MYPT3 interaction modulates the activity of both PP1beta and PP1gamma2. This is, to our knowledge, the first demonstration of a novel activity for an Oaz-encoded protein.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Oaz3Ratnegative regulation of stress fiber assembly  IDA  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Oaz3Ratsperm flagellum  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Oaz3Ratankyrin repeat binding  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Oaz3  (ornithine decarboxylase antizyme 3)


Additional Information