RGD Reference Report - Increased immunoreactivities against endothelin-converting enzyme-1 and monocyte chemotactic protein-1 in hepatic stellate cells of rat fibrous liver induced by thioacetamide.

General

Increased immunoreactivities against endothelin-converting enzyme-1 and monocyte chemotactic protein-1 in hepatic stellate cells of rat fibrous liver induced by thioacetamide.
Authors:	Nagata, T Kudo, H Nishino, T Doi, Y Itoh, H Fujimoto, S
Citation:	Nagata T, etal., Med Mol Morphol. 2005 Sep;38(3):161-72.
RGD ID:	7243952
Pubmed:	PMID:16170464 (View Abstract at PubMed)
DOI:	DOI:10.1007/s00795-005-0292-5 (Journal Full-text)
The progression of rat liver fibrosis induced by intraperitoneal administration of thioacetamide (TAA) was evaluated by immunocytochemistry using anti-alpha-smooth muscle actin (alpha-SMA), antiendothelin-converting enzyme (ECE)-1, and anti-monocyte chemotactic protein (MCP)-1 antibodies. The fibrous septal spaces gradually increased after administration of TAA, and pseudolobules were established in the 7-week TAA-treated groups. Immunoreactivities against alpha-SMA were not detected in hepatic stellate cells (HSCs) of the control group without TAA treatment, although they were observed in the HSCs around the fibrous septal spaces in all TAA-treated groups, indicating that activation of HSCs occurs during the establishment of pseudolobules. Immunoreactivities against ECE-1 and MCP-1 were seen in such HSCs of the TAA-treated groups, but few or no immunoreactivities were detected in the HSCs of the control group. The most significant increase in the ECE-1 immunoreactivities was detected in the 1-week TAA-treated group, whereas that in MCP-1 was observed in the 7-week TAA-treated group. The present immunocytochemistry indicated a difference in the accelerated expression period between immunoreactivities against ECE-1 and MCP-1 in the HSCs during the progression of TAA-induced liver fibrosis, suggesting that ECE-1 is involved in the early phase of liver fibrosis and that MCP-1 plays a role during the later phase.

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
Experimental Liver Cirrhosis		ISO	Ece1 (Rattus norvegicus)	7243952; 7243952	protein:increased expression:liver	RGD
Experimental Liver Cirrhosis		IEP		7243952	protein:increased expression:liver	RGD

Objects Annotated

Genes (Rattus norvegicus)

Ece1 (endothelin converting enzyme 1)

Genes (Mus musculus)

Ece1 (endothelin converting enzyme 1)

Genes (Homo sapiens)

ECE1 (endothelin converting enzyme 1)

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Increased immunoreactivities against endothelin-converting enzyme-1 and monocyte chemotactic protein-1 in hepatic stellate cells of rat fibrous liver induced by thioacetamide.