RGD Reference Report - Regulation of renal organic anion and cation transporters by thymoquinone in cisplatin induced kidney injury. - Rat Genome Database

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Regulation of renal organic anion and cation transporters by thymoquinone in cisplatin induced kidney injury.

Authors: Ulu, R  Dogukan, A  Tuzcu, M  Gencoglu, H  Ulas, M  Ilhan, N  Muqbil, I  Mohammad, RM  Kucuk, O  Sahin, K 
Citation: Ulu R, etal., Food Chem Toxicol. 2012 May;50(5):1675-9. doi: 10.1016/j.fct.2012.02.082. Epub 2012 Mar 3.
RGD ID: 7243179
Pubmed: PMID:22414646   (View Abstract at PubMed)
DOI: DOI:10.1016/j.fct.2012.02.082   (Journal Full-text)

In previous studies, we have demonstrated the biological activity of thymoquinone (TQ), an active compound extracted from the Nigella sativa plant, against cisplatin-induced neurotoxicity. Recenty, it was observed that there is an inherent lack in regulation of renal organic anion and cation transporters in cisplatin-induced nephrotoxicity. Here, we report, for the first time, the effect of TQ on alterations in the renal expression of organic anion transporters (OATs) and organic cation transporters (OCTs), as well as multidrug resistance-associated proteins (MRPs) in rats treated with cisplatin. Twenty-eight 8-week-old male Wistar rats were divided into four groups of control, TQ treated (10 mg/kg b.w. in drinking water for 5 days), cisplatin (7 mg/kg b.w., i.p.) and TQ and cisplatin combination treatment. Cisplatin-induced malondialdehyde (MDA) and 8-isoprostane increase was found to be markedly reduced in rats treated with TQ. In cisplatin only treated rats, the induced renal injury increased protein levels of the efflux transporters MRP2 and MRP4 while expression of OAT1, OAT3, OCT1 and OCT2 was reduced. In combination TQ- and cisplatin-treated rats, expression of MRP2 and MRP4 proteins was decreased in the kidneys. Conversely, TQ treatment increased levels of OCT1, OCT2, OAT1 and OAT3 and decreased levels of 8-isoprostane and MDA levels in cisplatin-treated rats. In conclusion, the present study shows that the TQ synergizes with its nephroprotective effect against cisplatin-induced acute kidney injury in rats.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
SLC22A1Humanacute kidney failure treatmentISOSlc22a1 (Rattus norvegicus) RGD 
SLC22A2Humanacute kidney failure treatmentISOSlc22a2 (Rattus norvegicus) RGD 
Slc22a1Ratacute kidney failure treatmentIDA  RGD 
Slc22a1Mouseacute kidney failure treatmentISOSlc22a1 (Rattus norvegicus) RGD 
Slc22a2Ratacute kidney failure treatmentIDA  RGD 
Slc22a2Mouseacute kidney failure treatmentISOSlc22a2 (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc22a1  (solute carrier family 22 member 1)
Slc22a2  (solute carrier family 22 member 2)

Genes (Mus musculus)
Slc22a1  (solute carrier family 22 (organic cation transporter), member 1)
Slc22a2  (solute carrier family 22 (organic cation transporter), member 2)

Genes (Homo sapiens)
SLC22A1  (solute carrier family 22 member 1)
SLC22A2  (solute carrier family 22 member 2)


Additional Information