RGD Reference Report - Acute inflammatory proteins constitute the organic matrix of prostatic corpora amylacea and calculi in men with prostate cancer. - Rat Genome Database

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Acute inflammatory proteins constitute the organic matrix of prostatic corpora amylacea and calculi in men with prostate cancer.

Authors: Sfanos, KS  Wilson, BA  De Marzo, AM  Isaacs, WB 
Citation: Sfanos KS, etal., Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3443-8. doi: 10.1073/pnas.0810473106. Epub 2009 Feb 6.
RGD ID: 7243107
Pubmed: PMID:19202053   (View Abstract at PubMed)
PMCID: PMC2651291   (View Article at PubMed Central)
DOI: DOI:10.1073/pnas.0810473106   (Journal Full-text)

Corpora amylacea (CA) are a frequent microscopic finding in radical prostatectomy specimens from men undergoing treatment for prostate cancer. Although often observed histologically to be associated with inflammation, the contribution of CA to prostatitis-related symptoms of unknown etiology or to prostate carcinogenesis remains unclear. Prostatic calculi (PC), which potentially represent calcified forms of CA, are less common but can cause urological disease including urinary retention and prostatitis. We conducted a comprehensive compositional analysis of CA/PC to gain insight into their biogenesis. Infrared spectroscopy analysis of calculi collected from 23 patients confirmed a prevalence of calcium phosphate in the form of hydroxyapatite. This result sets PC apart from most urinary stones, which largely are composed of calcium oxalate. Tandem mass spectrometry-based proteomic analysis of CA/PC revealed that lactoferrin is the predominant protein component, a result that was confirmed by Western blot analysis. Other proteins identified, including calprotectin, myeloperoxidase, and alpha-defensins, are proteins contained in neutrophil granules. Immunohistochemistry (IHC) suggested the source of lactoferrin to be prostate-infiltrating neutrophils as well as inflamed prostate epithelium; however, IHC for calprotectin suggested prostate-infiltrating neutrophils as a major source of the protein, because it was absent from other prostate compartments. This study represents a definitive analysis of the protein composition of prostatic CA and calculi and suggests that acute inflammation has a role in their biogenesis--an intriguing finding, given the prevalence of CA in prostatectomy specimens and the hypothesized role for inflammation in prostate carcinogenesis.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
LTFHumanprostate calculus  IEP  RGD 
LtfRatprostate calculus  ISOLTF (Homo sapiens) RGD 
LtfMouseprostate calculus  ISOLTF (Homo sapiens) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ltf  (lactotransferrin)

Genes (Mus musculus)
Ltf  (lactotransferrin)

Genes (Homo sapiens)
LTF  (lactotransferrin)


Additional Information