RGD Reference Report - Pro-inflammatory cytokine interleukin-1beta promotes the development of intestinal stem cells. - Rat Genome Database

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Pro-inflammatory cytokine interleukin-1beta promotes the development of intestinal stem cells.

Authors: Wang, L  Liu, Z  Li, Y  Pappan, L  Galliher-Beckley, A  Shi, J 
Citation: Wang L, etal., Inflamm Res. 2012 Oct;61(10):1085-92. doi: 10.1007/s00011-012-0501-3. Epub 2012 Jun 16.
RGD ID: 7240518
Pubmed: PMID:22706317   (View Abstract at PubMed)
DOI: DOI:10.1007/s00011-012-0501-3   (Journal Full-text)

OBJECTIVE: We investigated the effect of IL-1beta on the development of intestinal epithelial stem cells. MATERIALS AND METHODS: Normal intestinal epithelial cell line IEC-18 cells were cultured in the presence or absence of 200 pM of IL-1beta in serum-free medium (SFM) for various time periods. The effects of IL-1beta on intestinal stem cell self-renewal and IEC-18 cell proliferation were evaluated by a colony formation assay, MTT assay, and a focus formation assay. The expression of stemness genes including Bmi-1, Lgr-5, c-myc, Nanog, and beta-catenin in IEC-18 cells were measured by quantitative PCR and western blot analysis. RESULTS: IEC-18 cells grew as a monolayer in SFM in the absence of IL-1beta. Cellular spheres were formed when IEC-18 cells were grown in SFM in the presence of IL-1beta. IL-1beta induced the development of large colonies in the soft-agar as well as the formation of foci when IEC-18 cells were cultured in type-I collagen-coated plates. The expression of Bmi-1, Lgr-5, c-myc, Nanog, and beta-catenin were significantly increased in IEC-18 cells treated with IL-1beta. CONCLUSION: Our studies provide direct evidence the IL-1beta may play an important role in the self-renewal of intestinal epithelial stem cells and the development of cancer stem cells.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to interleukin-1  IEP 7240518 RGD 
cellular response to interleukin-1  IEP 7240518IL-1betaRGD 

Objects Annotated

Genes (Rattus norvegicus)
Bmi1  (BMI1 proto-oncogene, polycomb ring finger)
Myc  (MYC proto-oncogene, bHLH transcription factor)


Additional Information