RGD Reference Report - Temperature dependence of T-type calcium channel gating. - Rat Genome Database

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Temperature dependence of T-type calcium channel gating.

Authors: Iftinca, M  McKay, BE  Snutch, TP  McRory, JE  Turner, RW  Zamponi, GW 
Citation: Iftinca M, etal., Neuroscience. 2006 Nov 3;142(4):1031-42. Epub 2006 Aug 28.
RGD ID: 7207257
Pubmed: PMID:16935432   (View Abstract at PubMed)
DOI: DOI:10.1016/j.neuroscience.2006.07.010   (Journal Full-text)

T-type calcium channel isoforms are expressed in a multitude of tissues and have a key role in a variety of physiological processes. To fully appreciate the physiological role of distinct channel isoforms it is essential to determine their kinetic properties under physiologically relevant conditions. We therefore characterized the gating behavior of expressed rat voltage-dependent calcium channels (Ca(v)) 3.1, Ca(v)3.2, and Ca(v)3.3, as well as human Ca(v)3.3 at 21 degrees C and 37 degrees C in saline that approximates physiological conditions. Exposure to 37 degrees C caused significant increases in the rates of activation, inactivation, and recovery from inactivation, increased the current amplitudes, and induced a hyperpolarizing shift of half-activation for Ca(v)3.1 and Ca(v)3.2. At 37 degrees C the half-inactivation showed a hyperpolarizing shift for Ca(v)3.1 and Ca(v)3.2 and human Ca(v)3.3, but not rat Ca(v)3.3. The observed changes in the kinetics were significant but not identical for the three isoforms, showing that the ability of T-type channels to conduct calcium varies with both channel isoform and temperature.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
calcium ion import  IDA 7207257; 7207257; 7207257 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
voltage-gated calcium channel complex  ICGO:00083327207257; 7207257; 7207257 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
low voltage-gated calcium channel activity  IDA 7207257; 7207257; 7207257 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cacna1g  (calcium voltage-gated channel subunit alpha1 G)
Cacna1h  (calcium voltage-gated channel subunit alpha1 H)
Cacna1i  (calcium voltage-gated channel subunit alpha1 I)


Additional Information