RGD Reference Report - [Activation of calcium-sensing receptors is associated with apoptosis in cardiomyocytes under simulated ischemia/reperfusion]. - Rat Genome Database

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[Activation of calcium-sensing receptors is associated with apoptosis in cardiomyocytes under simulated ischemia/reperfusion].

Authors: Yan, L  Zhu, TB  Sun, TT  Pan, SY  Wang, LS  Tao, ZX  Yang, ZJ  Cao, KJ 
Citation: Yan L, etal., Zhejiang Da Xue Xue Bao Yi Xue Ban. 2011 Mar;40(2):207-12.
RGD ID: 7205700
Pubmed: PMID:21488219   (View Abstract at PubMed)

OBJECTIVE: To examine the association of activation of calcium-sensing receptors (CaSR) with apoptosis in cardiomyocytes under simulated ischemia/reperfusion. METHODS: Ventricular cardiomyocytes of neonatal rats were incubated in ischemia-mimetic solution for 2 h, then re-incubated in normal culture medium for 24 h to establish a model of simulated ischemia/reperfusion (I/R). Cell apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL assay). The expression of CaSR mRNA was detected by reverse transcriptase polymerase chain reaction (RT-PCR). The expression of Caspase -3 and Bcl-2 was detected by Western blotting. RESULT: The simulated I/R enhanced the expression of CaSR and cardiomyocyte apoptosis. GdCl(3), a specific activator of CaSR, further increased the expression of CaSR and cardiomyocyte apoptosis, along with upregulation of Caspase-3 and downregulation of Bcl-2. CONCLUSION: CaSR is associated with I/R injury and apoptosis in neonatal rat ventricular cardiomyocytes via suppressing Bcl-2 and promoting Caspase -3 expression.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to ischemia  IEP 7205700 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Casr  (calcium-sensing receptor)


Additional Information