RGD Reference Report - Release mode of large and small dense-core vesicles specified by different synaptotagmin isoforms in PC12 cells. - Rat Genome Database

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Release mode of large and small dense-core vesicles specified by different synaptotagmin isoforms in PC12 cells.

Authors: Zhang, Z  Wu, Y  Wang, Z  Dunning, FM  Rehfuss, J  Ramanan, D  Chapman, ER  Jackson, MB 
Citation: Zhang Z, etal., Mol Biol Cell. 2011 Jul 1;22(13):2324-36. doi: 10.1091/mbc.E11-02-0159. Epub 2011 May 5.
RGD ID: 7205660
Pubmed: PMID:21551071   (View Abstract at PubMed)
PMCID: PMC3128534   (View Article at PubMed Central)
DOI: DOI:10.1091/mbc.E11-02-0159   (Journal Full-text)

Many cells release multiple substances in different proportions according to the specific character of a stimulus. PC12 cells, a model neuroendocrine cell line, express multiple isoforms of the exocytotic Ca(2+) sensor synaptotagmin. We show that these isoforms sort to populations of dense-core vesicles that differ in size. These synaptotagmins differ in their Ca(2+) sensitivities, their preference for full fusion or kiss-and-run, and their sensitivity to inhibition by synaptotagmin IV. In PC12 cells, vesicles that harbor these different synaptotagmin isoforms can be preferentially triggered to fuse by different forms of stimulation. The mode of fusion is specified by the synaptotagmin isoform activated, and because kiss-and-run exocytosis can filter small molecules through a size-limiting fusion pore, the activation of isoforms that favor kiss-and-run will select smaller molecules over larger molecules packaged in the same vesicle. Thus synaptotagmin isoforms can provide multiple levels of control in the release of different molecules from the same cell.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Syt1Ratdense core granule  IDA  RGD 
Syt4Ratdense core granule  IDA  RGD 
Syt7Ratdense core granule  IDA  RGD 
Syt9Ratdense core granule  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Syt1  (synaptotagmin 1)
Syt4  (synaptotagmin 4)
Syt7  (synaptotagmin 7)
Syt9  (synaptotagmin 9)


Additional Information