RGD Reference Report - Distribution of the Ca2+-binding S100A1 protein at different sarcomere lengths of slow and fast rat skeletal muscles. - Rat Genome Database

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Distribution of the Ca2+-binding S100A1 protein at different sarcomere lengths of slow and fast rat skeletal muscles.

Authors: Maco, B  Uhrik, B  Heizmann, CW 
Citation: Maco B, etal., Gen Physiol Biophys. 2000 Jun;19(2):237-44.
RGD ID: 7205634
Pubmed: PMID:11156445   (View Abstract at PubMed)

The localization of S100A1 in rat soleus (SOL) and extensor digitorum longus (EDL) muscles was studied immunocytochemically at different sarcomere lengths (stretched, relaxed and contracted) at the ultrastructural level. The muscle fibres were contracted by application of 15 mmol/l caffeine. Following aldehyde fixation, dehydration and embedding in Lowicryl HM20 (-35 degrees C) ultrathin sections were incubated with rabbit polyclonal antiserum against S100A1. Goat antirabbit secondary antibodies conjugated with 10 nm gold particles were used to visualize antigen sites. Relative areas of Z-lines, A- and I-bands were estimated from longitudinal sections by the point counting method. The highest densities of the particles were found at the Z-lines. A higher incidence of S100A1 antigen sites in I-bands than in A-bands and a higher density of S100A1 in lateral parts of A-bands (with actin and myosin filaments overlapping) compared with the central area of A-bands are consistent with an interaction of S100A1 with F-actin in skeletal muscles. Antigen sites were also present at M-lines and at distinct locations of the sarcoplasmic reticulum.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
S100a1RatA band  IDA  RGD 
S100a1RatI band  IDA  RGD 
S100a1RatM band  IDA  RGD 
S100a1RatZ disc  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
S100a1  (S100 calcium binding protein A1)


Additional Information