RGD Reference Report - Proteomic analysis of rat plasma following transient focal cerebral ischemia. - Rat Genome Database
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Proteomic analysis of rat plasma following transient focal cerebral ischemia.

Authors: Chen, R  Vendrell, I  Chen, CP  Cash, D  O'Toole, KG  Williams, SA  Jones, C  Preston, JE  Wheeler, JX 
Citation: Chen R, etal., Biomark Med. 2011 Dec;5(6):837-46.
RGD ID: 7175514
Pubmed: (View Article at PubMed) PMID:22103620
DOI: Full-text: DOI:10.2217/bmm.11.89

AIM: This study aimed to identify plasma protein changes in a rat model of ischemic stroke using a proteomic approach. MATERIALS & METHODS: Four male Sprague-Dawley rats (3-6 months old) were subjected to 90 min of left middle cerebral artery occlusion under anesthesia with 1.5% isoflurane in O(2)/air followed by 24-h reperfusion. Blood samples (~100 microl) were collected at baseline, at the end of 90-min middle cerebral artery occlusion and at 24-h postreperfusion. Brain injuries were assessed by MRI at 24-h postreperfusion. Quantitative comparison of global plasma protein expression was performed using 2D differential in-gel electrophoresis. Differentially expressed protein spots were identified using peptide sequencing tandem mass spectrometry. RESULTS: These rats had clear brain infarction in the left hemisphere detected by MRI. Thirty-three protein spots of plasma samples were differentially expressed following focal cerebral ischemia/reperfusion. These protein spots belonged to eight proteins. Six of them (alpha2-macroglobulin, complement C3, inter-alpha- trypsin inhibitor heavy chain H3, serum albumin, haptoglobin and transthyretin), which are a class of acute-phase proteins, changed significantly. CONCLUSION: This study describes the responses of young rats to focal cerebral ischemia and suggests that future studies should use aged animals to better mimic the clinical ischemic stroke setting.


Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
C3  (complement C3)
Hp  (haptoglobin)

Genes (Mus musculus)
C3  (complement component 3)
Hp  (haptoglobin)

Genes (Homo sapiens)
C3  (complement C3)
HP  (haptoglobin)

Additional Information