RGD Reference Report - Clinical features of patients with anti-neutrophil cytoplasmic autoantibodies targeting native myeloperoxidase antigen. - Rat Genome Database

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Clinical features of patients with anti-neutrophil cytoplasmic autoantibodies targeting native myeloperoxidase antigen.

Authors: Yamanishi, Y  Ito-Ihara, T  Nagao, T  Uno, K  Kobayashi, S  Muso, E  Shane, PY  Firestein, GS  Hashimoto, H  Okazaki, T  Suzuki, K 
Citation: Yamanishi Y, etal., Mod Rheumatol. 2012 Oct 21.
RGD ID: 7174703
Pubmed: PMID:23085883   (View Abstract at PubMed)
DOI: DOI:10.1007/s10165-012-0781-z   (Journal Full-text)

OBJECTIVES: Anti-neutrophil cytoplasmic autoantibodies (ANCA) are useful diagnostic markers in systemic vasculitic disorders with small-vessel involvement, but depending on the particular test used, the myeloperoxidase (MPO)-ANCA results are variable. In the present study, we performed a comparative analysis between our originally developed nMPO-ANCA assay that targets the native MPO antigen and other commercially available assays using sera of patients with clinical features of ANCA-associated vasculitis (AAV). METHODS: Sera of 24 patients strongly suspected of having AAV were examined for the presence of MPO-ANCAs by our nMPO-ANCA assay and by other commercial-based MPO-ANCA assays. These results were correlated to indirect immunofluorescence microscopy staining patterns and patient clinical parameters. RESULTS: Eighteen out of 24 patients (75 %) were positive for nMPO-ANCA, compared with 13 out of 24 patients (54 %) by one of the most frequently used commercial-based MPO-ANCA enzyme-linked immunosorbent assays (ELISAs) in Japan. Interestingly, the patients who tested positive with our nMPO-ANCA assay alone showed clinical features of AAV marked by continuous fever, polyarthritis, and mild nephritis. The titers of nMPO-ANCA decreased in association with clinical improvement after treatment. CONCLUSIONS: Our data suggest that a positive nMPO-ANCA result, which identifies antibodies to human native MPO antigen, correlates with AAV disease activity. Moreover, the nMPO-ANCA test has clinical utility in detecting AAV-affected patients who have tested negative using commercially available assays.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis disease_progressionIEP 7174703 RGD 
Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis disease_progressionISOMPO (Homo sapiens)7174703; 7174703 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mpo  (myeloperoxidase)

Genes (Mus musculus)
Mpo  (myeloperoxidase)

Genes (Homo sapiens)
MPO  (myeloperoxidase)


Additional Information