RGD Reference Report - Molecular cloning and expression of a rat cDNA encoding 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase. - Rat Genome Database

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Molecular cloning and expression of a rat cDNA encoding 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase.

Authors: Akira, T  Komatsu, M  Nango, R  Tomooka, A  Konaka, K  Yamauchi, M  Kitamura, Y  Nomura, S  Tsukamoto, I 
Citation: Akira T, etal., Gene 1997 Sep 15;197(1-2):289-93.
RGD ID: 70804
Pubmed: PMID:9332377   (View Abstract at PubMed)

The cDNA of a 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (AICARFT/IMPCHase) was isolated from rat liver RNA by reverse transcription and the polymerase chain reaction (PCR). The rat AICARFT/IMPCHase cDNA included 1928 bp containing a coding region of 1779 bp for a 592-amino acid polypeptide (Mr = 64 200). Rat and human AICARFT/IMPCHase cDNAs show 84 and 91% homology at the nucleotide and amino acid sequence level, respectively. The protein produced by the rat cDNA using pET-expression system catalysed the penultimate and final steps of de novo purine biosynthesis. Northern analysis identified a 2.8-kb AICARFT/IMPCHase mRNA and the level of the AICARFT/IMPCHase transcripts increased markedly at 24 h after partial (70%) hepatectomy.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AticRat'de novo' IMP biosynthetic process  IDA  RGD 
AticRatanimal organ regeneration  IEP  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AticRatIMP cyclohydrolase activity  IDA  RGD 
AticRatphosphoribosylaminoimidazolecarboxamide formyltransferase activity  IDA  RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations


  
Objects Annotated

Genes (Rattus norvegicus)
Atic  (5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase)

Genes (Mus musculus)
Atic  (5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase)

Genes (Homo sapiens)
ATIC  (5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase)


Additional Information