RGD Reference Report - Expression of NAD(+)-dependent isocitrate dehydrogenase in brown adipose tissue. - Rat Genome Database

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Expression of NAD(+)-dependent isocitrate dehydrogenase in brown adipose tissue.

Authors: Shinohara, Y  Daikoku, T  Kajimoto, K  Shima, A  Yamazaki, N  Terada, H 
Citation: Shinohara Y, etal., Biochem Biophys Res Commun 2001 Mar 2;281(3):634-8.
RGD ID: 70770
Pubmed: PMID:11237704   (View Abstract at PubMed)
DOI: DOI:10.1006/bbrc.2001.4351   (Journal Full-text)

cDNA clones significantly expressed in brown adipose tissue (BAT) but not in white adipose tissue (WAT) of rats were isolated by use of a PCR-select cDNA subtraction kit. Of the isolated clones, structural features of two of them, 2-58 and 2-67, were studied in detail. The results indicated that these clones were cDNAs encoding alpha- and beta-subunits of rat NAD(+)-dependent isocitrate dehydrogenase (NAD(+)-ICDH). Previous biochemical study suggested the importance of NAD(+)-ICDH in metabolism in BAT; however, transcript levels of individual subunits of this enzyme in BAT had never been analyzed. In the present study, using these newly isolated cDNAs, we clearly demonstrate that the expression of three subunits of NAD(+)-ICDH was the most remarkable in BAT among the various tissues analyzed.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
isocitrate dehydrogenase (NAD+) activity  IDA 70770 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Idh3a  (isocitrate dehydrogenase (NAD(+)) 3 catalytic subunit alpha)

Objects referenced in this article
Gene Idh3b isocitrate dehydrogenase (NAD(+)) 3 non-catalytic subunit beta Rattus norvegicus

Additional Information