RGD Reference Report - Citron binds to PSD-95 at glutamatergic synapses on inhibitory neurons in the hippocampus. - Rat Genome Database

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Citron binds to PSD-95 at glutamatergic synapses on inhibitory neurons in the hippocampus.

Authors: Zhang, W  Vazquez, L  Apperson, M  Kennedy, MB 
Citation: Zhang W, etal., J Neurosci 1999 Jan 1;19(1):96-108.
RGD ID: 70684
Pubmed: PMID:9870942   (View Abstract at PubMed)
PMCID: PMC6782379   (View Article at PubMed Central)

Synaptic NMDA-type glutamate receptors are anchored to the second of three PDZ (PSD-95/Discs large/ZO-1) domains in the postsynaptic density (PSD) protein PSD-95. Here, we report that citron, a protein target for the activated form of the small GTP-binding protein Rho, preferentially binds the third PDZ domain of PSD-95. In GABAergic neurons from the hippocampus, citron forms a complex with PSD-95 and is concentrated at the postsynaptic side of glutamatergic synapses. Citron is expressed only at low levels in glutamatergic neurons in the hippocampus and is not detectable at synapses onto these neurons. In contrast to citron, p135 SynGAP, an abundant synaptic Ras GTPase-activating protein that can bind to all three PDZ domains of PSD-95, and Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) are concentrated postsynaptically at glutamatergic synapses on glutamatergic neurons. CaM kinase II is not expressed and p135 SynGAP is expressed in less than half of hippocampal GABAergic neurons. Segregation of citron into inhibitory neurons does not occur in other brain regions. For example, citron is expressed at high levels in most thalamic neurons, which are primarily glutamatergic and contain CaM kinase II. In several other brain regions, citron is present in a subset of neurons that can be either GABAergic or glutamatergic and can sometimes express CaM kinase II. Thus, in the hippocampus, signal transduction complexes associated with postsynaptic NMDA receptors are different in glutamatergic and GABAergic neurons and are specialized in a way that is specific to the hippocampus.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
gamma-aminobutyric acid signaling pathway  IDA 70684 RGD 
postsynaptic cytoskeleton organization  IDA 70684 RGD 
postsynaptic density protein 95 clustering  IDA 70684 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
GABA-ergic synapse  IDA 70684MMO:0000665 more ...RGD 
glutamatergic postsynaptic density  IDA 70684MMO:0000665 more ...RGD 
postsynaptic density, intracellular component  IDA 70684MMO:0000665 more ...RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
scaffold protein binding  IPIDlg4 (Rattus norvegicus)70684 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cit  (citron rho-interacting serine/threonine kinase)


Additional Information