RGD Reference Report - Ischemia-induced association of the stress protein alpha B-crystallin with I-band portion of cardiac titin. - Rat Genome Database

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Ischemia-induced association of the stress protein alpha B-crystallin with I-band portion of cardiac titin.

Authors: Golenhofen, N  Arbeiter, A  Koob, R  Drenckhahn, D 
Citation: Golenhofen N, etal., J Mol Cell Cardiol 2002 Mar;34(3):309-19.
RGD ID: 704398
Pubmed: PMID:11945023   (View Abstract at PubMed)
DOI: DOI:10.1006/jmcc.2001.1513   (Journal Full-text)

During ischemia the cardiac stress protein, alpha B-crystallin, was shown by immunoelectron microscopy to translocate to the N(2)-line area of myofibrillar I-bands of rat cardiomyocytes where alpha B-crystallin resisted extraction with 1 m NaSCN and 2 m urea as did titin. Actin became completely extracted under these conditions, indicating association of alpha B-crystallin with titin the only remaining non-actin cytoskeletal component of I-bands outside Z-disks. Titin, extracted from ischemic pig myocardium, was shown to copurify with alpha B-crystallin. Further evidence for binding of alpha B-crystallin to titin was obtained by dot-blot assays in which biotinylated alpha B-crystallin was demonstrated to bind to the titin-enriched fraction immobilized on nitrocellulose. Binding of alpha B-crystallin to titin during cardiac ischemia could serve to stabilize titin against denaturation and might provide an endogenous mechanism to delay ischemic damage of this important elastic component of myofibrils.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Myocardial Ischemia  IDA 704398 RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
I band  IDA 704398 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cryab  (crystallin, alpha B)


Additional Information