RGD Reference Report - A novel brain-specific isoform of beta spectrin: isolation and its interaction with Munc13. - Rat Genome Database

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A novel brain-specific isoform of beta spectrin: isolation and its interaction with Munc13.

Authors: Sakaguchi, G  Orita, S  Naito, A  Maeda, M  Igarashi, H  Sasaki, T  Takai, Y 
Citation: Sakaguchi G, etal., Biochem Biophys Res Commun 1998 Jul 30;248(3):846-51.
RGD ID: 70020
Pubmed: PMID:9704016   (View Abstract at PubMed)
DOI: DOI:10.1006/bbrc.1998.9067   (Journal Full-text)

Munc13 is a component of the neurotransmitter release machinery which is specifically expressed in brain. Munc13 interacts with Doc2 and syntaxin which are also implicated in the neurotransmitter release process. Here we isolated another Munc13-interacting molecule from a rat brain cDNA library by use of the yeast two-hybrid system, identified it to be a novel type of beta spectrin, and named it beta SpIII sigma 1. beta SpIII sigma 1 was specifically expressed in brain, where it was enriched in the synaptic vesicle and plasma membrane fractions. Because spectrin has been shown to interact with the actin cytoskeleton which is involved in the exocytotic process, the present results suggest that the Munc13-beta SpIII sigma 1 interactions play a role in neurotransmitter release.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Sptbn2Ratregulation of calcium ion-dependent exocytosis involved_inTAS PMID:9704016UniProt 
Sptbn2Ratsynaptic vesicle docking involved_inTAS PMID:9704016UniProt 

Cellular Component

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Sptbn2Ratcytoplasm located_inTAS PMID:9704016UniProt 
Sptbn2Ratnuclear matrix located_inTAS PMID:9704016UniProt 
Sptbn2Ratspectrin part_ofTAS PMID:9704016UniProt 
Sptbn2Ratsynaptic vesicle  IDA  RGD 

Molecular Function

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Sptbn2Ratprotein binding  IPIUnc13a (Rattus norvegicus) RGD 
Unc13aRatspectrin binding  IPISptbn2 (Rattus norvegicus) RGD 
Sptbn2Ratstructural constituent of cytoskeleton enablesTAS PMID:9704016UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Sptbn2  (spectrin, beta, non-erythrocytic 2)
Unc13a  (unc-13 homolog A)

Additional Information