RGD Reference Report - Identification, cloning, and sequencing of a cDNA coding for rat gamma-glutamyl hydrolase. - Rat Genome Database

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Identification, cloning, and sequencing of a cDNA coding for rat gamma-glutamyl hydrolase.

Authors: Yao, R  Nimec, Z  Ryan, TJ  Galivan, J 
Citation: Yao R, etal., J Biol Chem 1996 Apr 12;271(15):8525-8.
RGD ID: 69845
Pubmed: (View Article at PubMed) PMID:8621474

Purified gamma-glutamyl hydrolase secreted from rat H35 hepatoma cells has been characterized as a diffuse band of 55 kDa on SDS-polyacrylamide gel electrophoresis that is converted to bands of 35 and 33 kDa after enzymatic removal of N-linked carbohydrate. Polyclonal antibodies against 55-kDa gamma-glutamyl hydrolase captured the enzyme activity and recognized the glycosylated and both deglycosylated forms of gamma-glutamyl hydrolase. A complete cDNA sequence of gamma-glutamyl hydrolase was obtained using degenerate oligonucleotides derived from peptide sequences, screening of a rat hepatoma cDNA library, and reverse transcription polymerase chain reaction. Based upon the deduced amino acid sequence the peptide component of gamma-glutamyl hydrolase had a molecular weight of 33,400. The results of amino acid analysis of the purified protein agreed with the deduced amino acid sequence in which there are seven potential asparagine-containing glycosylation sites.



Gene Ontology Annotations    

Biological Process

Cellular Component

Molecular Function

Molecular Pathway Annotations    
Objects Annotated

Genes (Rattus norvegicus)
Ggh  (gamma-glutamyl hydrolase)

Genes (Mus musculus)
Ggh  (gamma-glutamyl hydrolase)

Genes (Homo sapiens)
GGH  (gamma-glutamyl hydrolase)


Additional Information