RGD Reference Report - Requirement of a 5-lipoxygenase-activating protein for leukotriene synthesis. - Rat Genome Database

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Requirement of a 5-lipoxygenase-activating protein for leukotriene synthesis.

Authors: Dixon, RA  Diehl, RE  Opas, E  Rands, E  Vickers, PJ  Evans, JF  Gillard, JW  Miller, DK 
Citation: Dixon RA, etal., Nature 1990 Jan 18;343(6255):282-4.
RGD ID: 69749
Pubmed: PMID:2300173   (View Abstract at PubMed)
DOI: DOI:10.1038/343282a0   (Journal Full-text)

Leukotrienes, the biologically active metabolites of arachidonic acid, have been implicated in a variety of inflammatory responses, including asthma, arthritis and psoriasis. Recently a compound, MK-886, has been described that blocks the synthesis of leukotrienes in intact activated leukocytes, but has little or no effect on enzymes involved in leukotriene synthesis, including 5-lipoxygenase, in cell-free systems. A membrane protein with a high affinity for MK-886 and possibly representing the cellular target for MK-886 has been isolated from rat and human leukocytes. Here, we report the isolation of a complementary DNA clone encoding the MK-886-binding protein. We also demonstrate that the expression of both the MK-886-binding protein and 5-lipoxygenase is necessary for leukotriene synthesis in intact cells. Because the MK-886-binding protein seems to play a part in activating this enzyme in cells, it is termed the five-lipoxygenase activating protein (FLAP).



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Alox5apRatleukotriene biosynthetic process  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Alox5apRatarachidonate 5-lipoxygenase activity contributes_toIDA  RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations


  
Objects Annotated

Genes (Rattus norvegicus)
Alox5ap  (arachidonate 5-lipoxygenase activating protein)

Genes (Mus musculus)
Alox5ap  (arachidonate 5-lipoxygenase activating protein)

Genes (Homo sapiens)
ALOX5AP  (arachidonate 5-lipoxygenase activating protein)


Additional Information