RGD Reference Report - A novel arachidonate-preferring acyl-CoA synthetase is present in steroidogenic cells of the rat adrenal, ovary, and testis. - Rat Genome Database

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A novel arachidonate-preferring acyl-CoA synthetase is present in steroidogenic cells of the rat adrenal, ovary, and testis.

Authors: Kang, MJ  Fujino, T  Sasano, H  Minekura, H  Yabuki, N  Nagura, H  Iijima, H  Yamamoto, TT 
Citation: Kang MJ, etal., Proc Natl Acad Sci U S A 1997 Apr 1;94(7):2880-4.
RGD ID: 69374
Pubmed: PMID:9096315   (View Abstract at PubMed)
PMCID: PMC20291   (View Article at PubMed Central)

We report herein the cDNA cloning of a novel rat acyl-CoA synthetase (ACS) that preferentially uses arachidonate and eicosapentaenoate. This newly identified ACS (designated ACS4) contains 670 amino acids and is 68% identical to rat ACS3, a previously characterized ACS that is highly expressed in brain. ACS4 was overproduced in Escherichia coli and the resulting enzyme was purified to homogeneity. The purified enzyme utilizes arachidonate and eicosapentaenoate most preferentially among C8-C22 saturated fatty acids and C14-C22 unsaturated fatty acids. Kinetic analyses revealed that the enzyme has a high affinity for arachidonate and eicosapentaenoate and low affinity for palmitate. ACS4 transcripts are detectable in a wide range of tissues, with the highest level in adrenal gland. Immunoreactivity to ACS4 was detected in the zona fasciculata and reticularis of adrenal gland, in the corpus luteum and stromal luteinized cells in ovary, and in the Leydig cells of testis.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Acsl4Ratfatty acid metabolic process  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Acsl4Ratlong-chain fatty acid-CoA ligase activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Acsl4  (acyl-CoA synthetase long-chain family member 4)


Additional Information