RGD Reference Report - Properties and tissue distribution of a novel aldo-keto reductase encoding in a rat gene (Akr1b10). - Rat Genome Database

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Properties and tissue distribution of a novel aldo-keto reductase encoding in a rat gene (Akr1b10).

Authors: Endo, S  Matsunaga, T  Kuragano, T  Ohno, S  Kitade, Y  Tajima, K  El-Kabbani, O  Hara, A 
Citation: Endo S, etal., Arch Biochem Biophys. 2010 Nov 15;503(2):230-7. Epub 2010 Aug 13.
RGD ID: 6903952
Pubmed: PMID:20709016   (View Abstract at PubMed)
DOI: DOI:10.1016/j.abb.2010.08.010   (Journal Full-text)

A recent rat genomic sequencing predicts a gene Akr1b10 that encodes a protein with 83% sequence similarity to human aldo-keto reductase (AKR) 1B10. In this study, we isolated the cDNA for the rat AKR1B10 (R1B10) from rat brain, and examined the enzymatic properties of the recombinant protein. R1B10 utilized NADPH as the preferable coenzyme, and reduced various aldehydes (including cytotoxic 4-hydroxy-2-hexenal and 4-hydroxy- and 4-oxo-2-nonenals) and alpha-dicarbonyl compounds (such as methylglyoxal and 3-deoxyglucosone), showing low K(m) values of 0.8-6.1muM and 3.7-67muM, respectively. The enzyme also reduced glyceraldehyde and tetroses (K(m)=96-390muM), although hexoses and pentoses were inactive and poor substrates, respectively. Among the substrates, 4-oxo-2-nonenal was most efficiently reduced into 4-oxo-2-nonenol, and its cytotoxicity against bovine endothelial cells was decreased by the overexpression of R1B10. R1B10 showed low sensitivity to aldose reductase inhibitors, and was activated to approximately two folds by valproic acid, and alicyclic and aromatic carboxylic acids. The mRNA for R1B10 was expressed highly in rat brain and heart, and at low levels in other rat tissues and skin fibroblasts. The results suggest that R1B10 functions as a defense system against oxidative stress and glycation in rat tissues.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Akr1b10Ratpolyol metabolic process  IDA  RGD 
Akr1b10Ratretinal metabolic process  IDA  RGD 
Akr1b10Ratretinol metabolic process  IDA  RGD 

Molecular Function

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Akr1b10Rataldose reductase (NADPH) activity  IDA  RGD 
Akr1b10Ratall-trans-retinol dehydrogenase (NADP+) activity  IDA  RGD 
Akr1b10RatNADP+ binding  IDA  RGD 
Akr1b10RatNADPH binding  IDA  RGD 
Akr1b10Ratretinal binding  IDA  RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AKR1B10Humanretinol metabolic pathway   ISOAkr1b10 (Rattus norvegicus) RGD 
Akr1b10Ratretinol metabolic pathway   IDA  RGD 
Akr1b10Mouseretinol metabolic pathway   ISOAkr1b10 (Rattus norvegicus) RGD 
Objects Annotated

Genes (Rattus norvegicus)
Akr1b10  (aldo-keto reductase family 1 member B10)

Genes (Mus musculus)
Akr1b10  (aldo-keto reductase family 1, member B10)

Genes (Homo sapiens)
AKR1B10  (aldo-keto reductase family 1 member B10)

Additional Information