RGD Reference Report - Localizations of visual cycle components in retinal pigment epithelium. - Rat Genome Database

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Localizations of visual cycle components in retinal pigment epithelium.

Authors: Huang, J  Possin, DE  Saari, JC 
Citation: Huang J, etal., Mol Vis. 2009;15:223-34. Epub 2009 Jan 26.
RGD ID: 6893662
Pubmed: PMID:19180257   (View Abstract at PubMed)
PMCID: PMC2632734   (View Article at PubMed Central)

PURPOSE: We used immunocytochemistry and confocal microscopy to determine whether enzymes of the rod visual cycle were uniformly distributed in retinal pigment epithelium (RPE) cells. The localizations of these enzymes were compared to known localizations of retinoid-binding proteins and associated proteins. METHODS: Antibodies to proteins and enzymes associated with the rod visual cycle were used for fluorescence immunocytochemistry with frozen sections of albino mouse and rat retina. Images were obtained with a laser scanning confocal microscope. RESULTS: Components associated with the rod visual cycle were distributed in three distinct patterns in mouse and rat RPE. Three visual cycle enzymes (RDH5, LRAT, and RPE65) were restricted to the somata of RPE cells and were not detected within apical processes. Ezrin, an actin-binding protein, and ERM-binding phosphoprotein50/sodium-hydrogen exchanger regulatory factor1 (EBP50/NHERF1), an ezrin-binding PDZ-domain protein, were largely restricted to RPE apical processes. The fluorescence intensity over Muller cell apical processes was less intense. Cellular retinaldehyde-binding protein (CRALBP), which binds to EBP50/NHERF1, and cellular retinol-binding protein type 1 (CRBP1) were found throughout RPE cells and Muller cells. CONCLUSIONS: Visual cycle enzymes were confined to the somata of RPE cells and did not occur within the long apical processes, either in dark- or light-adapted animals. Other components previously linked to the visual cycle (EBP50/NHERF1 and ezrin) were largely confined to the apical processes, where they could be associated with release of 11-cis-retinal or uptake of all-trans-retinol. CRALBP and CRBP1 were distributed throughout the RPE cell, where they could mediate diffusion of retinoids between apical processes and somata.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cell body  IDA 6893662; 6893662; 6893662; 6893662 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Rbp1  (retinol binding protein 1)
Rdh5  (retinol dehydrogenase 5)
Rlbp1  (retinaldehyde binding protein 1)
Rpe65  (retinoid isomerohydrolase RPE65)


Additional Information