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Application of thrombelastography in liver injury induced by endotoxin in rat.

Authors: Tsai, HJ  Tsao, CM  Liao, MH  Ka, SM  Liaw, WJ  Wu, CC 
Citation: Tsai HJ, etal., Blood Coagul Fibrinolysis. 2012 Mar;23(2):118-26. doi: 10.1097/MBC.0b013e32834ee170.
Pubmed: (View Article at PubMed) PMID:22227956
DOI: Full-text: DOI:10.1097/MBC.0b013e32834ee170

Liver injury developing in patients with sepsis may lead to an increased risk of mortality. Thrombelastography (TEG) is generally applied to evaluate hemostatic disturbance in patients undergoing liver transplantation or cardiopulmonary bypass. The aim of this study was to investigate the development of liver injury and coagulopathy in a lipopolysaccharide (LPS)-induced animal model and to assess the relationship between TEG variables and liver injury. Male Wistar rats received LPS (30 mg/kg over a 4-h intravenous infusion) to induce experimental liver injury or isotonic saline as a control. Variables of hemodynamics and liver biochemistry were measured during the subsequent 6 h after the start of infusion. TEG variables (R-time, K-time, alpha-angle and maximal amplitude), thrombin-antithrombin complex and plasminogen activator inhibitor-1 were also measured. After LPS infusion, liver injury [examined by biochemical variables (e.g. alanine aminotransferase, ALT) and histological studies] was developed and inflammatory cytokines (tumor necrosis factor-alpha and interleukin-6) were raised. At the initial period of LPS infusion, R-time was shortened and alpha-angle was increased. Thereafter, alpha-angle and maximal amplitude were decreased progressively, demonstrating that endotoxin induced coagulation disturbances. Furthermore, there were strong positive correlation between K-time and natural log (Ln)(ALT) (r = 0.823, P = 0.001); also, there were strong negative correlations between alpha-angle and Ln(ALT) (r = -0.762, P = 0.002) as well as maximal amplitude and Ln(ALT) (r = -0.732, P = 0.004) at 6 h after LPS infusion. These results demonstrated that TEG could be a potential tool to evaluate the development of liver injury in endotoxemia.


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RGD Object Information
RGD ID: 6893482
Created: 2012-08-31
Species: All species
Last Modified: 2012-08-31
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.