RGD Reference Report - Cloning and characterization of the thyroid iodide transporter. - Rat Genome Database

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Cloning and characterization of the thyroid iodide transporter.

Authors: Dai, G  Levy, O  Carrasco, N 
Citation: Dai G, etal., Nature 1996 Feb 1;379(6564):458-60.
RGD ID: 68854
Pubmed: (View Article at PubMed) PMID:8559252
DOI: Full-text: DOI:10.1038/379458a0

Iodide (I-) is an essential constituent of the thyroid hormones T3 and T4, and is accumulated by the thyroid. The transport of iodide, the first step in thyroid hormogenesis, is catalysed by the Na+/I- symporter, an intrinsic membrane protein that is crucial for the evaluation, diagnosis and treatment of thyroid disorders. Although several other important thyroid proteins involved in hormogenesis have been characterized, the Na+/I- symporter has not. Here we report the isolation of a complementary DNA clone that encodes this symporter, as a result of functional screening of a cDNA library from a rat thyroid-derived cell line (FRTL-5) in Xenopus laevis oocytes. Oocyte microinjection of an RNA transcript made in vitro from this cDNA clone elicited a more than 700-fold increase in perchlorate-sensitive Na+/I- symport activity over background. To our knowledge, this is the first iodide-transporting molecule to have its cDNA cloned, providing a missing link in the thyroid hormone biosynthetic pathway.

Annotation

Gene Ontology Annotations    

Biological Process

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Slc5a5  (solute carrier family 5 member 5)


Additional Information