RGD Reference Report - Cloning and crystal structure of hematopoietic prostaglandin D synthase. - Rat Genome Database

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Cloning and crystal structure of hematopoietic prostaglandin D synthase.

Authors: Kanaoka, Y  Ago, H  Inagaki, E  Nanayama, T  Miyano, M  Kikuno, R  Fujii, Y  Eguchi, N  Toh, H  Urade, Y  Hayaishi, O 
Citation: Kanaoka Y, etal., Cell 1997 Sep 19;90(6):1085-95.
RGD ID: 68838
Pubmed: PMID:9323136   (View Abstract at PubMed)

Hematopoietic prostaglandin (PG) D synthase is the key enzyme for production of the D and J series of prostanoids in the immune system and mast cells. We isolated a cDNA for the rat enzyme, crystallized the recombinant enzyme, and determined the three-dimensional structure of the enzyme complexed with glutathione at 2.3 A resolution. The enzyme is the first member of the sigma class glutathione S-transferase (GST) from vertebrates and possesses a prominent cleft as the active site, which is never seen among other members of the GST family. The unique 3-D architecture of the cleft leads to the putative substrate binding mode and its catalytic mechanism, responsible for the specific isomerization from PGH2 to PGD2.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
HpgdsRatprostaglandin biosynthetic process  TAS  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
HpgdsRatprostaglandin-D synthase activity  TAS  RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations


  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
HPGDSHumanprostanoid metabolic pathway  ISOHpgds (Rattus norvegicus) RGD 
HpgdsRatprostanoid metabolic pathway  IDA  RGD 
HpgdsMouseprostanoid metabolic pathway  ISOHpgds (Rattus norvegicus) RGD 
Objects Annotated

Genes (Rattus norvegicus)
Hpgds  (hematopoietic prostaglandin D synthase)

Genes (Mus musculus)
Hpgds  (hematopoietic prostaglandin D synthase)

Genes (Homo sapiens)
HPGDS  (hematopoietic prostaglandin D synthase)


Additional Information