RGD Reference Report - Immunolocalization of retinoic acid biosynthesis systems in selected sites in rat. - Rat Genome Database

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Immunolocalization of retinoic acid biosynthesis systems in selected sites in rat.

Authors: Everts, HB  Sundberg, JP  Ong, DE 
Citation: Everts HB, etal., Exp Cell Res. 2005 Aug 15;308(2):309-19.
RGD ID: 6771322
Pubmed: (View Article at PubMed) PMID:15950969
DOI: Full-text: DOI:10.1016/j.yexcr.2005.04.026

Vitamin A deficiency leads to focal metaplasia of numerous epithelial tissues with altered differentiation from columnar (in general) to stratified squamous cells. This process can be reversed with vitamin A repletion. Previously, we described a system of retinoic acid (RA) synthesis in the cycling rat uterus consisting of cellular retinol binding protein (Crbp), epithelial retinol dehydrogenase (eRoldh), retinal dehydrogenase 2 (Aldh1a2), and cellular retinoic acid binding protein type II (Crabp2). Western blot analysis, RT-PCR, and immunohistochemistry were performed to test whether this retinoic acid synthesis system was also present in other vitamin A sensitive tissues. We found that combinations of Crbp, eRoldh, Aldh1a2 or Aldh1a3, and Crabp2 were present in all vitamin A sensitive tissues examined. In the ureter, while eRoldh was present, another short chain alcohol dehydrogenase reductase (possibly Roldh 1, 2, or 3) was in higher concentration in the transitional epithelia. In several tissues, Crbp, Aldh1a2, and/or Aldh1a3 localized to mesenchyme and/or epithelial cells, while eRoldh and Crabp2 were expressed only in epithelial cells. This suggests that mesenchymal-epithelial interactions may be as important in the adult as they are during development and that local synthesis of RA is important in maintenance of these tissues.

Gene Ontology Annotations    

Biological Process

Molecular Pathway Annotations    
Objects Annotated

Genes (Rattus norvegicus)
Aldh1a2  (aldehyde dehydrogenase 1 family, member A2)
Aldh1a3  (aldehyde dehydrogenase 1 family, member A3)
Crabp2  (cellular retinoic acid binding protein 2)
Dhrs9  (dehydrogenase/reductase 9)
Rbp1  (retinol binding protein 1)

Genes (Mus musculus)
Aldh1a2  (aldehyde dehydrogenase family 1, subfamily A2)
Aldh1a3  (aldehyde dehydrogenase family 1, subfamily A3)
Crabp2  (cellular retinoic acid binding protein II)
Dhrs9  (dehydrogenase/reductase (SDR family) member 9)
Rbp1  (retinol binding protein 1, cellular)

Genes (Homo sapiens)
ALDH1A2  (aldehyde dehydrogenase 1 family member A2)
ALDH1A3  (aldehyde dehydrogenase 1 family member A3)
CRABP2  (cellular retinoic acid binding protein 2)
DHRS9  (dehydrogenase/reductase 9)
RBP1  (retinol binding protein 1)

Additional Information