RGD Reference Report - Immunolocalization of retinoic acid biosynthesis systems in selected sites in rat. - Rat Genome Database

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Immunolocalization of retinoic acid biosynthesis systems in selected sites in rat.

Authors: Everts, HB  Sundberg, JP  Ong, DE 
Citation: Everts HB, etal., Exp Cell Res. 2005 Aug 15;308(2):309-19.
RGD ID: 6771322
Pubmed: PMID:15950969   (View Abstract at PubMed)
DOI: DOI:10.1016/j.yexcr.2005.04.026   (Journal Full-text)

Vitamin A deficiency leads to focal metaplasia of numerous epithelial tissues with altered differentiation from columnar (in general) to stratified squamous cells. This process can be reversed with vitamin A repletion. Previously, we described a system of retinoic acid (RA) synthesis in the cycling rat uterus consisting of cellular retinol binding protein (Crbp), epithelial retinol dehydrogenase (eRoldh), retinal dehydrogenase 2 (Aldh1a2), and cellular retinoic acid binding protein type II (Crabp2). Western blot analysis, RT-PCR, and immunohistochemistry were performed to test whether this retinoic acid synthesis system was also present in other vitamin A sensitive tissues. We found that combinations of Crbp, eRoldh, Aldh1a2 or Aldh1a3, and Crabp2 were present in all vitamin A sensitive tissues examined. In the ureter, while eRoldh was present, another short chain alcohol dehydrogenase reductase (possibly Roldh 1, 2, or 3) was in higher concentration in the transitional epithelia. In several tissues, Crbp, Aldh1a2, and/or Aldh1a3 localized to mesenchyme and/or epithelial cells, while eRoldh and Crabp2 were expressed only in epithelial cells. This suggests that mesenchymal-epithelial interactions may be as important in the adult as they are during development and that local synthesis of RA is important in maintenance of these tissues.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Aldh1a2Ratretinoic acid biosynthetic process  IEP  RGD 
Aldh1a3Ratretinoic acid biosynthetic process  IEP  RGD 
Crabp2Ratretinoic acid biosynthetic process  IEP  RGD 
Dhrs9Ratretinoic acid biosynthetic process  IEP  RGD 
Rbp1Ratretinoic acid biosynthetic process  IEP  RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations


  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
ALDH1A2Humanretinoic acid metabolic pathway   ISORGD:620250 RGD 
ALDH1A3Humanretinoic acid metabolic pathway   ISORGD:628662 RGD 
Aldh1a2Mouseretinoic acid metabolic pathway   ISORGD:620250 RGD 
Aldh1a2Ratretinoic acid metabolic pathway   IEP  RGD 
Aldh1a3Mouseretinoic acid metabolic pathway   ISORGD:628662 RGD 
Aldh1a3Ratretinoic acid metabolic pathway   IEP  RGD 
CRABP2Humanretinoic acid metabolic pathway   ISORGD:62070 RGD 
Crabp2Mouseretinoic acid metabolic pathway   ISORGD:62070 RGD 
Crabp2Ratretinoic acid metabolic pathway   IEP  RGD 
DHRS9Humanretinoic acid metabolic pathway   ISORGD:620655 RGD 
Dhrs9Mouseretinoic acid metabolic pathway   ISORGD:620655 RGD 
Dhrs9Ratretinoic acid metabolic pathway   IEP  RGD 
RBP1Humanretinoic acid metabolic pathway   ISORGD:3543 RGD 
Rbp1Ratretinoic acid metabolic pathway   IEP  RGD 
Rbp1Mouseretinoic acid metabolic pathway   ISORGD:3543 RGD 
Objects Annotated

Genes (Rattus norvegicus)
Aldh1a2  (aldehyde dehydrogenase 1 family, member A2)
Aldh1a3  (aldehyde dehydrogenase 1 family, member A3)
Crabp2  (cellular retinoic acid binding protein 2)
Dhrs9  (dehydrogenase/reductase 9)
Rbp1  (retinol binding protein 1)

Genes (Mus musculus)
Aldh1a2  (aldehyde dehydrogenase family 1, subfamily A2)
Aldh1a3  (aldehyde dehydrogenase family 1, subfamily A3)
Crabp2  (cellular retinoic acid binding protein II)
Dhrs9  (dehydrogenase/reductase 9)
Rbp1  (retinol binding protein 1, cellular)

Genes (Homo sapiens)
ALDH1A2  (aldehyde dehydrogenase 1 family member A2)
ALDH1A3  (aldehyde dehydrogenase 1 family member A3)
CRABP2  (cellular retinoic acid binding protein 2)
DHRS9  (dehydrogenase/reductase 9)
RBP1  (retinol binding protein 1)


Additional Information