RGD Reference Report - Isoliquiritigenin from licorice root suppressed neovascularisation in experimental ocular angiogenesis models. - Rat Genome Database

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Isoliquiritigenin from licorice root suppressed neovascularisation in experimental ocular angiogenesis models.

Authors: Jhanji, V  Liu, H  Law, K  Lee, VY  Huang, SF  Pang, CP  Yam, GH 
Citation: Jhanji V, etal., Br J Ophthalmol. 2011 Sep;95(9):1309-15. Epub 2011 Jun 30.
RGD ID: 6771213
Pubmed: (View Article at PubMed) PMID:21719569
DOI: Full-text: DOI:10.1136/bjophthalmol-2011-300110

AIM: To explore the antiangiogenic property of isoliquiritigenin (ISL) on in vivo and in vitro models. DESIGN: Laboratory investigation. METHODS: The effect of ISL on angiogenesis development was investigated using ex ovo chick chorioallantoic membrane model. Its effect on pathological angiogenesis was examined by (1) silver nitrate cauterisation-induced corneal neovascularisation in BALB/c mice, followed by topical ISL (0.2-50 muM) and CD31 immunofluorescence of corneal blood vessels; (2) argon laser photocoagulation-induced choroidal neovascularisation in C57BL/6 mice, followed by intravitreal ISL (10-200 muM) and fundus fluorescein angiography and immunofluorescence with Griffonia simplicifolia isolectin-B4 (GSA I-B4); and (3) oxygen-induced retinopathy in C57BL/6J mice pups, followed by intravitreal ISL (1-100 muM) and GSA I-B4 immunofluorescence. The vascular area was quantified and analysed by one-way analysis of variance and Student t test. Expression of vascular endothelial growth factor (VEGF) and pigment-epithelium-derived factor in human umbilical vein endothelial cells was analysed by western blotting. RESULTS: Ex ovo chick chorioallantoic membrane assay showed that ISL dose-dependently suppressed VEGF-induced vessel growth. In vivo experiments illustrated that topical ISL alleviated corneal neovascularisation (IC(50)=7.14 muM, day 7) and intravitreal ISL reduced vessel leakage and GSA I-B4-positive vascular area in choroidal and retinal neovascularisation. ISL was found to dose-dependently suppress VEGF and induce pigment epithelium derived factor expression in cultured endothelial cells. CONCLUSION: Using various experimental models of ocular neovascularisation, the authors have demonstrated that ISL from licorice extract has an antiangiogenic effect. The authors' findings suggest that ISL may be a potential antiangiogenic molecule in the development of therapy for neovascularisation diseases.

Annotation

Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Pecam1  (platelet and endothelial cell adhesion molecule 1)

Genes (Mus musculus)
Pecam1  (platelet/endothelial cell adhesion molecule 1)

Genes (Homo sapiens)
PECAM1  (platelet and endothelial cell adhesion molecule 1)


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