RGD Reference Report - Expression of antioxidant molecules after peripheral nerve injury and regeneration. - Rat Genome Database

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Expression of antioxidant molecules after peripheral nerve injury and regeneration.

Authors: Lanza, C  Raimondo, S  Vergani, L  Catena, N  Senes, F  Tos, P  Geuna, S 
Citation: Lanza C, etal., J Neurosci Res. 2012 Apr;90(4):842-8. doi: 10.1002/jnr.22778. Epub 2012 Jan 18.
RGD ID: 6483815
Pubmed: PMID:22253198   (View Abstract at PubMed)
DOI: DOI:10.1002/jnr.22778   (Journal Full-text)

Oxidative stress is considered to be one of the main causes of neural damage after injury. However, little is known about the changes in mRNA expression of antioxidant molecules that occur after injury and regeneration of the peripheral nerve. In the present study, the rat median nerve was transected, and transcriptional changes were studied at day 6 and day 12 after injury in both the proximal and the distal stumps, in the absence or presence of microsurgical repair. The expression profiles of the following genes were investigated: three metallothionein isoforms (MT-1, MT-2, and MT-3), the main antioxidant enzymes (catalase, superoxide dismutase, and glutathione-S-transferase), and the marker of cellular damage poly(ADP-ribose) polymerase-1 (PARP-1). The results showed that, in the proximal nerve stump, MT-3 mRNA expression was significantly and markedly up-regulated in the absence of surgical repair, whereas MT-1 and MT-2 showed significant down-regulation. In the distal nerve portion, mRNA expression of all MT isoforms decreased significantly in the absence of microsurgical reconstruction, whereas, after repair, MT-3 mRNA expression alone was up-regulated. Expression of all the antioxidant enzymes decreased significantly after repair in the proximal nerve portion, but a significant general increase in their mRNA expression was revealed in the distal nerve stump. PARP-1 expression was significantly up-regulated in the proximal nerve portion without repair but dramatically reduced after reconstruction. In contrast, PARP-1 expression increased markedly in the distal stump after surgical repair. Taken together, these findings indicate that antioxidant molecules are differentially modulated and might, therefore, play an important role in peripheral nerve injury and regeneration.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MT1FHumanmedian neuropathy  ISOMt1 (Rattus norvegicus) RGD 
MT2AHumanmedian neuropathy  ISOMt2A (Rattus norvegicus)mRNA:decreased expression:left median nerve (rat)RGD 
MT3Humanmedian neuropathy treatmentISOMt3 (Rattus norvegicus) RGD 
Mt1Ratmedian neuropathy  IEP  RGD 
Mt1Mousemedian neuropathy  ISOMt1 (Rattus norvegicus) RGD 
Mt2Mousemedian neuropathy  ISOMt2A (Rattus norvegicus)mRNA:decreased expression:left median nerve (rat)RGD 
Mt2ARatmedian neuropathy  IEP mRNA:decreased expression:left median nerve (rat)RGD 
Mt3Ratmedian neuropathy treatmentIEP  RGD 
Mt3Mousemedian neuropathy treatmentISOMt3 (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mt1  (metallothionein 1)
Mt2A  (metallothionein 2A)
Mt3  (metallothionein 3)

Genes (Mus musculus)
Mt1  (metallothionein 1)
Mt2  (metallothionein 2)
Mt3  (metallothionein 3)

Genes (Homo sapiens)
MT1F  (metallothionein 1F)
MT2A  (metallothionein 2A)
MT3  (metallothionein 3)


Additional Information