RGD Reference Report - Monocyte chemotactic protein-3: possible involvement in apical periodontitis chemotaxis. - Rat Genome Database

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Monocyte chemotactic protein-3: possible involvement in apical periodontitis chemotaxis.

Authors: Dezerega, A  Osorio, C  Mardones, J  Mundi, V  Dutzan, N  Franco, M  Gamonal, J  Oyarzun, A  Overall, CM  Hernandez, M 
Citation: Dezerega A, etal., Int Endod J. 2010 Oct;43(10):902-8. doi: 10.1111/j.1365-2591.2010.01764.x. Epub 2010 Jul 15.
RGD ID: 6483772
Pubmed: (View Article at PubMed) PMID:20646081
DOI: Full-text: DOI:10.1111/j.1365-2591.2010.01764.x

AIM: To study the expression of monocyte chemotactic protein-3 (MCP-3, also known as chemokine CCL-7) in tissue from apical lesions (AL) and to associate MCP-3 expression with symptomatic or asymptomatic apical periodontitis. METHODOLOGY: To determine the expression of MCP-3 in AL, biopsies obtained during tooth extraction procedures were fixed, subjected to routine processing and diagnosed as apical granuloma (AG) (n = 7) or radicular cyst (RC) (n = 5). As controls, apical periodontal ligament (PDL) specimens from healthy premolars extracted for orthodontics reasons were included (n = 7). All specimens were immunostained for MCP-3 and examined under a light microscope. In addition, homogenates from AL (n = 14) and healthy PDL samples (n = 7) were studied through immunowestern blot. Finally, periapical exudates samples were collected from root canals of teeth having diagnosis of symptomatic (n = 14) and asymptomatic apical periodontitis (n = 14) during routine endodontic treatments and analysed by immunowestern blot and densitometry. RESULTS: MCP-3 was detected in AG and RC and localized mainly to inflammatory leucocytes, whereas no expression was observed in healthy PDLs. MCP-3 was also detected in periapical exudate, and its levels were significantly higher in symptomatic than in asymptomatic apical periodontitis. CONCLUSIONS: MCP-3 was expressed in AL and its levels associated with clinical symptoms. MCP-3 might play a role in disease pathogenesis, possibly by stimulating mononuclear chemotaxis.


Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Ccl7  (C-C motif chemokine ligand 7)

Genes (Mus musculus)
Ccl7  (chemokine (C-C motif) ligand 7)

Genes (Homo sapiens)
CCL7  (C-C motif chemokine ligand 7)

Additional Information