RGD Reference Report - Insulin-like growth factor I treatment reduces demyelination and up-regulates gene expression of myelin-related proteins in experimental autoimmune encephalomyelitis. - Rat Genome Database

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Insulin-like growth factor I treatment reduces demyelination and up-regulates gene expression of myelin-related proteins in experimental autoimmune encephalomyelitis.

Authors: Yao, DL  Liu, X  Hudson, LD  Webster, HD 
Citation: Yao DL, etal., Proc Natl Acad Sci U S A. 1995 Jun 20;92(13):6190-4.
RGD ID: 6483353
Pubmed: PMID:7541143   (View Abstract at PubMed)
PMCID: PMC41668   (View Article at PubMed Central)

To compare effects of insulin-like growth factor I (IGF-I) and placebo treatment on lesions that resemble those seen during active demyelination in multiple sclerosis, we induced experimental autoimmune encephalomyelitis in Lewis rats with an emulsion containing guinea pig spinal cord and Freund's adjuvant. On day 12-13, pairs of rats with the same degree of weakness were given either IGF-I or placebo intravenously twice daily for 8 days. After 8 days of placebo or IGF-I (200 micrograms/day or 1 mg/day) treatment, the spinal cord lesions were studied by in situ hybridization and with immunocytochemical and morphological methods. IGF-I produced significant reductions in numbers and areas of demyelinating lesions. These lesions contained axons surrounded by regenerating myelin segments instead of demyelinated axons seen in the placebo-treated rats. Relative mRNA levels for myelin basic protein, proteolipid protein (PLP), and 2',3'-cyclic nucleotide 3'-phosphodiesterase in lesions of IGF-I-treated rats were significantly higher than they were in placebo-treated rats. PLP mRNA-containing oligodendroglia also were more numerous and relative PLP mRNA levels per oligodendrocyte were higher in lesions of IGF-I-treated rats. Finally, a significantly higher proportion of proliferating cells were oligodendroglia-like cells in lesions of IGF-I-treated rats. We think that IGF-I effects on oligodendrocytes, myelin protein synthesis, and myelin regeneration reduced lesion severity and promoted clinical recovery in this experimental autoimmune encephalomyelitis model. These IGF-I actions may also benefit patients with multiple sclerosis.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Experimental Autoimmune Encephalomyelitis  ISOCnp (Rattus norvegicus)6483353; 6483353 RGD 
Experimental Autoimmune Encephalomyelitis  IDA 6483353 RGD 
Experimental Autoimmune Encephalomyelitis treatmentIDA 6483353human protein in a rat modelRGD 
Experimental Autoimmune Encephalomyelitis treatmentISOIGF1 (Homo sapiens)6483353; 6483353human protein in a rat modelRGD 

Objects Annotated

Genes (Rattus norvegicus)
Cnp  (2',3'-cyclic nucleotide 3' phosphodiesterase)
Igf1  (insulin-like growth factor 1)

Genes (Mus musculus)
Cnp  (2',3'-cyclic nucleotide 3' phosphodiesterase)
Igf1  (insulin-like growth factor 1)

Genes (Homo sapiens)
CNP  (2',3'-cyclic nucleotide 3' phosphodiesterase)
IGF1  (insulin like growth factor 1)


Additional Information