RGD Reference Report - NMDA receptor activation suppresses microtubule growth and spine entry. - Rat Genome Database

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NMDA receptor activation suppresses microtubule growth and spine entry.

Authors: Kapitein, LC  Yau, KW  Gouveia, SM  Van der Zwan, WA  Wulf, PS  Keijzer, N  Demmers, J  Jaworski, J  Akhmanova, A  Hoogenraad, CC 
Citation: Kapitein LC, etal., J Neurosci. 2011 Jun 1;31(22):8194-209.
RGD ID: 6483329
Pubmed: PMID:21632941   (View Abstract at PubMed)
PMCID: PMC6622869   (View Article at PubMed Central)
DOI: DOI:10.1523/JNEUROSCI.6215-10.2011   (Journal Full-text)

Dynamic microtubules are important to maintain neuronal morphology and function, but whether neuronal activity affects the organization of dynamic microtubules is unknown. Here, we show that a protocol to induce NMDA-dependent long-term depression (LTD) rapidly attenuates microtubule dynamics in primary rat hippocampal neurons, removing the microtubule-binding protein EB3 from the growing microtubule plus-ends in dendrites. This effect requires the entry of calcium and is mediated by activation of NR2B-containing NMDA-type glutamate receptor. The rapid NMDA effect is followed by a second, more prolonged response, during which EB3 accumulates along MAP2-positive microtubule bundles in the dendritic shaft. MAP2 is both required and sufficient for this activity-dependent redistribution of EB3. Importantly, NMDA receptor activation suppresses microtubule entry in dendritic spines, whereas overexpression of EB3-GFP prevents NMDA-induced spine shrinkage. These results suggest that short-lasting and long-lasting changes in dendritic microtubule dynamics are important determinants for NMDA-induced LTD.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein binding  IPIMAPRE3 (Homo sapiens)6483329 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Map2  (microtubule-associated protein 2)


Additional Information