RGD Reference Report - Bone cells from patients with quiescent Crohn's disease show a reduced growth potential and an impeded maturation. - Rat Genome Database

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Bone cells from patients with quiescent Crohn's disease show a reduced growth potential and an impeded maturation.

Authors: Oostlander, AE  Bravenboer, N  Van Essen, HW  Klein-Nulend, J  Lems, WF  Schulten, BA  Dijkstra, G  Van der Woude, CJ  Van Bodegraven, AA  Lips, P  Lips, Paul 
Citation: Oostlander AE, etal., J Cell Biochem. 2012 Jul;113(7):2424-2431. doi: 10.1002/jcb.24119.
RGD ID: 6483321
Pubmed: PMID:22535626   (View Abstract at PubMed)
DOI: DOI:10.1002/jcb.24119   (Journal Full-text)

Patients with Crohn's disease (CD) are at increased risk of developing osteoporosis. The mechanism underlying bone loss in CD patients is only partly understood. Inflammation is thought to contribute by causing a disturbed bone remodeling. In this study, we aimed to compare functional characteristics of osteoblasts from CD patients and controls, as osteoblasts are one of the effector cells in bone remodeling. The study included 18 patients with quiescent CD and 18 healthy controls. Bone cells obtained from iliac crest biopsies were cultured in the absence and presence of the inflammatory cytokines IL-1alpha, IL-1beta, IL-6, TNF-alpha, IL-10, and TGF-beta. At various time points, cell proliferation and differentiation were analyzed. Bone cells from CD patients showed a prolonged culture period to reach confluence and a decreased cell number at confluence. CD patient-derived bone cell cultures produced higher alkaline phosphatase levels, whereas osteocalcin levels were considerably reduced compared to control cultures. At the proliferation level, the responsiveness to inflammatory cytokines was similar in bone cells from CD patients and controls. At the differentiation level, CD cultures showed an increased responsiveness to IL-6 and a decreased responsiveness to TGF-beta. Responsiveness to the other cytokines tested was unaffected. In summary, we show a reduced growth potential and impeded maturation of bone cells from quiescent CD patients in vitro. These disease-related alterations combined with an unchanged sensitivity of CD patient-derived bone cells to inflammatory cytokines, provide a new insight in the understanding of CD-associated bone loss. J. Cell. Biochem. 113: 2424-2431, 2012. (c) 2012 Wiley Periodicals, Inc.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Crohn's disease  IEP 6483321mRNA:decreased expression:boneRGD 
Crohn's disease  ISOBGLAP (Homo sapiens)6483321; 6483321mRNA:decreased expression:boneRGD 

Objects Annotated

Genes (Rattus norvegicus)
Bglap  (bone gamma-carboxyglutamate protein)

Genes (Mus musculus)
Bglap  (bone gamma carboxyglutamate protein)

Genes (Homo sapiens)
BGLAP  (bone gamma-carboxyglutamate protein)


Additional Information