RGD Reference Report - Hypoxia modulates gene expression of IP3 receptors in rodent cerebellum. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models   new Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources

Hypoxia modulates gene expression of IP3 receptors in rodent cerebellum.

Authors: Jurkovicova, D  Kopacek, J  Stefanik, P  Kubovcakova, L  Zahradnikova A, JR  Zahradnikova, A  Pastorekova, S  Krizanova, O 
Citation: Jurkovicova D, etal., Pflugers Arch. 2007 Jun;454(3):415-25. Epub 2007 Feb 7.
RGD ID: 6482794
Pubmed: PMID:17285299   (View Abstract at PubMed)
DOI: DOI:10.1007/s00424-007-0214-6   (Journal Full-text)

Hypoxic brain cell injury is a complex process that results from a series of intracellular events. In this work, we tested whether severe hypoxia for 6 h can affect gene expression and protein levels of intracellular calcium channels, ryanodine receptors, and inositol 1,4,5-trisphosphate receptors in mouse cerebellum. In addition, we tested the effect of hypoxia on cerebellar granular cells of rats. We have found that gene expression of types 1 and 2 IP(3) receptors is significantly increased after the exposure of mice to hypoxic stimulus for 6 h and also in rat cerebellar granular cells. Increased gene expression of IP(3) receptors was reflected in increased protein levels of these channels as well. In this process, reactive oxygen species are most probably involved, as antioxidant quercetin abolished hypoxia-induced increase in both types 1 and 2 IP3 receptor. Ryanodine receptors of types 1 and 2 and sarco(endo)plasmic reticulum Ca(2+)-ATPase were not affected by hypoxia on the level of messenger RNA. To test physiological consequences, we measured levels of intracellular calcium. We observed significantly elevated calcium level in hypoxic compared to normoxic cells. Deeper understanding of mechanisms, through which hypoxia regulates intracellular calcium, could point towards the development of new therapeutic approaches to reduce or suppress the pathological effects of cellular hypoxia, such as those seen in stroke or ischemia.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Brain Hypoxia  ISOItpr1 (Mus musculus)6482794; 6482794mRNA and protein:increased expression:cerebellum (mouse)RGD 
Brain Hypoxia  ISOItpr2 (Mus musculus)6482794; 6482794mRNA and protein:increased expression:cerebellum (mouse)RGD 
Brain Hypoxia  IEP 6482794; 6482794mRNA and protein:increased expression:cerebellum (mouse)RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to hypoxia  IEP 6482794; 6482794 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Itpr1  (inositol 1,4,5-trisphosphate receptor, type 1)
Itpr2  (inositol 1,4,5-trisphosphate receptor, type 2)

Genes (Mus musculus)
Itpr1  (inositol 1,4,5-trisphosphate receptor 1)
Itpr2  (inositol 1,4,5-triphosphate receptor 2)

Genes (Homo sapiens)
ITPR1  (inositol 1,4,5-trisphosphate receptor type 1)
ITPR2  (inositol 1,4,5-trisphosphate receptor type 2)


Additional Information