RGD Reference Report - Altered gene expression in leukocyte transendothelial migration and cell communication pathways in periodontitis-affected gingival tissues. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models   new Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources

Altered gene expression in leukocyte transendothelial migration and cell communication pathways in periodontitis-affected gingival tissues.

Authors: Abe, D  Kubota, T  Morozumi, T  Shimizu, T  Nakasone, N  Itagaki, M  Yoshie, H 
Citation: Abe D, etal., J Periodontal Res. 2011 Jun;46(3):345-53. doi: 10.1111/j.1600-0765.2011.01349.x. Epub 2011 Mar 7.
RGD ID: 6480655
Pubmed: PMID:21382035   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1600-0765.2011.01349.x   (Journal Full-text)

BACKGROUND AND OBJECTIVE: Gene expression is related to the pathogenesis of periodontitis and plays a crucial role in local tissue destruction and disease susceptibility. The aims of the present study were to identify the expression of specific genes and biological pathways in periodontitis-affected gingival tissue using microarray and quantitative real-time RT-PCR analyses. MATERIAL AND METHODS: Healthy and periodontitis-affected gingival tissues were taken from three patients with severe chronic periodontitis. Total RNAs from six gingival tissue samples were used for microarray analyses. Data-mining analyses, such as comparisons, gene ontology and pathway analyses, were performed and biological pathways with a significant role in periodontitis were identified. In addition, quantitative real-time RT-PCR analysis was performed on samples obtained from 14 patients with chronic periodontitis and from 14 healthy individuals in order to confirm the results of the pathway analysis. RESULTS: Comparison analyses found 15 up-regulated and 13 down-regulated genes (all of which showed a change of more than twofold in expression levels) in periodontitis-affected gingival tissues. Pathway analysis identified 15 up-regulated biological pathways, including leukocyte transendothelial migration, and five down-regulated pathways, including cell communication. Quantitative real-time RT-PCR verified that five genes in the leukocyte transendothelial migration pathway were significantly up-regulated, and four genes in the cell communication pathway were significantly down-regulated, which was consistent with pathway analysis. CONCLUSION: We identified up-regulated genes (ITGB-2, MMP-2, CXCL-12, CXCR-4 and Rac-2) and down-regulated genes (connexin, DSG-1, DSC-1 and nestin) in periodontitis-affected gingival tissues; these genes may be related to the stimulation of leukocyte transendothelial migration and to the the impairment of cell-to-cell communication in periodontitis.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Chronic Periodontitis  IEP 6480655; 6480655; 6480655; 6480655; 6480655mRNA:increased expression:gingival tissuesRGD 
Chronic Periodontitis  ISOCXCL12 (Homo sapiens)6480655; 6480655mRNA:increased expression:gingival tissues (human)RGD 
Chronic Periodontitis  ISOCXCR4 (Homo sapiens)6480655; 6480655mRNA:increased expression:gingival tissues (human)RGD 
Chronic Periodontitis  IEP 6480655; 6480655; 6480655mRNA:decreased expression:gingival tissuesRGD 
Chronic Periodontitis  ISODSC1 (Homo sapiens)6480655; 6480655mRNA:decreased expression:gingival tissues (human)RGD 
Chronic Periodontitis  ISODSG1 (Homo sapiens)6480655; 6480655mRNA:decreased expression:gingival tissues (human)RGD 
Chronic Periodontitis  ISOITGB2 (Homo sapiens)6480655; 6480655mRNA:increased expression:gingival tissues (human)RGD 
Chronic Periodontitis  ISOMMP2 (Homo sapiens)6480655; 6480655mRNA:increased expression:gingival tissues (human)RGD 
Chronic Periodontitis  ISONES (Homo sapiens)6480655; 6480655mRNA:decreased expression:gingival tissues (human)RGD 
Chronic Periodontitis  ISORAC2 (Homo sapiens)6480655; 6480655mRNA:increased expression:gingival tissues (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cxcl12  (C-X-C motif chemokine ligand 12)
Cxcr4  (C-X-C motif chemokine receptor 4)
Dsc1  (desmocollin 1)
Dsg1  (desmoglein 1)
Itgb2  (integrin subunit beta 2)
Mmp2  (matrix metallopeptidase 2)
Nes  (nestin)
Rac2  (Rac family small GTPase 2)

Genes (Mus musculus)
Cxcl12  (C-X-C motif chemokine ligand 12)
Cxcr4  (C-X-C motif chemokine receptor 4)
Dsc1  (desmocollin 1)
Dsg1a  (desmoglein 1 alpha)
Itgb2  (integrin beta 2)
Mmp2  (matrix metallopeptidase 2)
Nes  (nestin)
Rac2  (Rac family small GTPase 2)

Genes (Homo sapiens)
CXCL12  (C-X-C motif chemokine ligand 12)
CXCR4  (C-X-C motif chemokine receptor 4)
DSC1  (desmocollin 1)
DSG1  (desmoglein 1)
ITGB2  (integrin subunit beta 2)
MMP2  (matrix metallopeptidase 2)
NES  (nestin)
RAC2  (Rac family small GTPase 2)


Additional Information