RGD Reference Report - Proteomic analysis of circulating human monocytes in coronary artery disease. - Rat Genome Database

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Proteomic analysis of circulating human monocytes in coronary artery disease.

Authors: Poduri, A  Bahl, A  Talwar, KK  Khullar, M 
Citation: Poduri A, etal., Mol Cell Biochem. 2012 Jan;360(1-2):181-8. Epub 2011 Sep 22.
RGD ID: 6480617
Pubmed: PMID:21938407   (View Abstract at PubMed)
DOI: DOI:10.1007/s11010-011-1055-3   (Journal Full-text)

Monocytes play an important role in inflammation and atherosclerosis; however, the molecular details underlying these diverse functions are not completely understood. Proteomic analysis of monocytes can provide new insights into their biological role in coronary artery disease (CAD). Twenty angiographically confirmed male, CAD patients (>/=50% stenosis) attending cardiology clinic of Nehru Hospital, PGIMER, Chandigarh, and who were not receiving any lipid lowering therapy and 20 TMT negative subjects who served as controls were enrolled in the study. Circulating monocytes isolated from overnight fasting blood samples were analyzed by 2D gel electrophoresis (pH 4-7), and differentially expressed protein spots were subjected to mass spectrometry and identification of proteins. We observed 333 +/- 40 protein spots in monocytes from patients and 312 +/- 20 in controls; out of which 63 protein spots showed altered intensity in CAD patients. Thirteen spots showed fivefold increased and two protein spots showed fivefold decreased expression in CAD group as compared to control group, respectively. Two proteins showing decreased expression in monocytes from CAD patients were identified as: (i) glutathione transferase and (ii) heat shock protein 70 KDa. Proteins showing increased expression in CAD patients were identified as: (i) vimentin, (ii) mannose binding lectin receptor protein, and (iii) S100A8 calcium-binding protein. The results of our study offer identification of several proteins in monocytes which can provide new perspectives in role of monocytes in pathogenesis of atherosclerosis.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
coronary artery disease  IEP 6480617protein:increased expression:monocyteRGD 
coronary artery disease  ISOVIM (Homo sapiens)6480617; 6480617protein:increased expression:monocyteRGD 

Objects Annotated

Genes (Rattus norvegicus)
Vim  (vimentin)

Genes (Mus musculus)
Vim  (vimentin)

Genes (Homo sapiens)
VIM  (vimentin)


Additional Information