RGD Reference Report - Metallothionein-III knockout mice aggravates the neuronal damage after transient focal cerebral ischemia. - Rat Genome Database

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Metallothionein-III knockout mice aggravates the neuronal damage after transient focal cerebral ischemia.

Authors: Koumura, A  Hamanaka, J  Shimazawa, M  Honda, A  Tsuruma, K  Uchida, Y  Hozumi, I  Satoh, M  Inuzuka, T  Hara, H 
Citation: Koumura A, etal., Brain Res. 2009 Oct 6;1292:148-54. Epub 2009 Jul 25.
RGD ID: 6480529
Pubmed: PMID:19635467   (View Abstract at PubMed)
DOI: DOI:10.1016/j.brainres.2009.07.050   (Journal Full-text)

Metallothioneins (MTs) are metal-binding proteins and have four isoforms. MT-III was, at first, found in the brains of patients with Alzheimer's disease. MT-III exists mainly in the central nervous system, and the main effects are thought to be anti-oxidative and regulate zinc levels. In some previous reports, MT-III exhibited neuroprotective effects in various pathological situations, but its detailed effects are still unclear. In the present study, we examined neuronal damage after a middle cerebral artery occlusion (MCAO) in MT-III knockout (KO) mice to elucidate the relationship between MT-III and cerebral infarction. There was no significant difference in cerebral infarction after 24-h permanent MCAO between the wild-type and MT-III KO mice. On the other hand, after 2-h MCAO and 22-h reperfusion, cerebral infarction in the MT-III KO mice was aggravated compared with the wild-type mice. Furthermore, fatal rate of MT-III KO mice increased from 3 days after MCAO, and neurological deficits at 5 and 7 days after MCAO of MT-III KO mice were worse than those of wild-type. We examined terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining and the immunostaining of an oxidative stress marker, 8-hydroxy-2'-deoxyguanosine (8-OHdG), at 24 h after transient MCAO. In the penumbra lesion, the positive cell numbers in both staining assays were higher in the MT-III KO mice than those of the wild-type mice. These findings indicate that neuronal damage was aggravated by reperfusion injury in the MT-III KO mice compared with the wild-type mice, suggesting that MT-III plays anti-oxidative and neuroprotective roles in transient cerebral ischemia.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MT3HumanReperfusion Injury  ISOMt3 (Mus musculus) RGD 
Mt3RatReperfusion Injury  ISOMt3 (Mus musculus) RGD 
Mt3MouseReperfusion Injury  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mt3  (metallothionein 3)

Genes (Mus musculus)
Mt3  (metallothionein 3)

Genes (Homo sapiens)
MT3  (metallothionein 3)


Additional Information