RGD Reference Report - Homer1a is a core brain molecular correlate of sleep loss. - Rat Genome Database

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Homer1a is a core brain molecular correlate of sleep loss.

Authors: Maret, S  Dorsaz, S  Gurcel, L  Pradervand, S  Petit, B  Pfister, C  Hagenbuchle, O  O'Hara, BF  Franken, P  Tafti, M 
Citation: Maret S, etal., Proc Natl Acad Sci U S A. 2007 Dec 11;104(50):20090-5. Epub 2007 Dec 6.
RGD ID: 6480426
Pubmed: PMID:18077435   (View Abstract at PubMed)
PMCID: PMC2148427   (View Article at PubMed Central)
DOI: DOI:10.1073/pnas.0710131104   (Journal Full-text)

Sleep is regulated by a homeostatic process that determines its need and by a circadian process that determines its timing. By using sleep deprivation and transcriptome profiling in inbred mouse strains, we show that genetic background affects susceptibility to sleep loss at the transcriptional level in a tissue-dependent manner. In the brain, Homer1a expression best reflects the response to sleep loss. Time-course gene expression analysis suggests that 2,032 brain transcripts are under circadian control. However, only 391 remain rhythmic when mice are sleep-deprived at four time points around the clock, suggesting that most diurnal changes in gene transcription are, in fact, sleep-wake-dependent. By generating a transgenic mouse line, we show that in Homer1-expressing cells specifically, apart from Homer1a, three other activity-induced genes (Ptgs2, Jph3, and Nptx2) are overexpressed after sleep loss. All four genes play a role in recovery from glutamate-induced neuronal hyperactivity. The consistent activation of Homer1a suggests a role for sleep in intracellular calcium homeostasis for protecting and recovering from the neuronal activation imposed by wakefulness.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Sleep Deprivation  ISOJph3 (Mus musculus)6480426; 6480426mRNA:increased expression:brainRGD 
Sleep Deprivation  IEP 6480426; 6480426mRNA:increased expression:brainRGD 
Sleep Deprivation  ISOPtgs2 (Mus musculus)6480426; 6480426mRNA:increased expression:brainRGD 

Objects Annotated

Genes (Rattus norvegicus)
Jph3  (junctophilin 3)
Ptgs2  (prostaglandin-endoperoxide synthase 2)

Genes (Mus musculus)
Jph3  (junctophilin 3)
Ptgs2  (prostaglandin-endoperoxide synthase 2)

Genes (Homo sapiens)
JPH3  (junctophilin 3)
PTGS2  (prostaglandin-endoperoxide synthase 2)

Additional Information