RGD Reference Report - Expression of gamma-aminobutyric acid A receptor subunits alpha1, beta1, gamma2 mRNA in rats with hepatic encephalopathy. - Rat Genome Database

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Expression of gamma-aminobutyric acid A receptor subunits alpha1, beta1, gamma2 mRNA in rats with hepatic encephalopathy.

Authors: Li, XQ  Dong, L  Liu, ZH  Luo, JY 
Citation: Li XQ, etal., World J Gastroenterol. 2005 Jun 7;11(21):3319-22.
RGD ID: 6480237
Pubmed: PMID:15929193   (View Abstract at PubMed)
PMCID: PMC4316074   (View Article at PubMed Central)

AIM: To investigate the mRNA expression of gamma-aminobutyric acid A (GABA(A)) receptor subunits alpha(1), beta(1), gamma(2) in different parts of the brain of rats with hepatic encephalopathy. METHODS: Twelve adult male Sprague-Dawley rats were randomly divided into two groups: (1) hepatic encephalopathy model group (n = 6), which was induced by intraperitoneal injection of thioacetamide (TAA, 350 mg/kg) for three consecutive days; (2) control group (n = 6), in which the rats were treated with same dose of normal saline solution. After the freeze slice of cerebrum was made, in situ hybridization was used to detect the mRNA of GABAA receptor subunits alpha(1), beta(1), and gamma(2) in rat cerebral cortex, basal nuclei, substantia nigra and hippocampi. Image data were collected and analyzed quantitatively by QWin550CW model image signal gather and analysis system. RESULTS: In rats with hepatic encephalopathy, mRNA expression levels of GABA(A) receptor subunits alpha(1), beta(1) increased significantly in basal nuclei, substantia nigra pars compacta, substantia nigra pars reticularis and hippocampi (144.7+/-15.67/184.14+/-4.41, 60.61+/-33.66/113.07+/-32.44, 87.71+/- 21.25/128.40+/-18.85, 122.34+/-5.56/161.60+/-4.56, 123.29+/-5.21/140.65+/-4.15, 123.40+/-4.42/140.09+/-4.52, 124.76+/-4.18/140.09+/-4.12, 141.62+/-15.09/182.80 +/-5.20, 69.13+/-30.74/134.21+/-43.76, 87.87+/-25.16/151.01+/-19.49, 122.14+/-6.30 /162.33+/-3.92, 122.81+/-5.09/137.19+/-7.12, 123.00+/-4.63/138.11+/-5.92, 125.75 +/-2.43/138.81+/-6.10, P<0.01), but did not change in the cerebral cortex compared to the control group. Similar changes were found in the mRNA expression levels of GABA(A) receptor subunit gamma(2), which increased significantly in basal nuclei, substantia nigra pars compacta, substantia nigra pars reticularis (136.81+/-26.41/167.97+/-16.23, 51.00+/-36.14/113.18+/-36.52, 86.35+/-20.30/ 126.90+/-19.74, P<0.01), CA1 of hippocampal (162.15+/-9.05/178.62+/-6.45, P<0.05), and no changes were found in the cerebral cortex and CA2, CA3, CA4 of hippocampi. CONCLUSION: In rats with hepatic encephalopathy, mRNA expression levels of GABA(A) receptor subunits alpha(1), beta(1), gamma(2) increase significantly in basal nuclei, substantia nigra and hippocampi, suggesting that the changes of mRNA expression levels in GABA(A) receptor subunits may contribute to the pathogenesis of hepatic encephalopathy.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
GABRA1Humanhepatic encephalopathy  ISOGabra1 (Rattus norvegicus)mRNA:increased expression:brainRGD 
GABRB1Humanhepatic encephalopathy  ISOGabrb1 (Rattus norvegicus)mRNA:increased expression:brainRGD 
GABRG2Humanhepatic encephalopathy  ISOGabrg2 (Rattus norvegicus)mRNA:increased expression:brainRGD 
Gabra1Rathepatic encephalopathy  IEP mRNA:increased expression:brainRGD 
Gabra1Mousehepatic encephalopathy  ISOGabra1 (Rattus norvegicus)mRNA:increased expression:brainRGD 
Gabrb1Rathepatic encephalopathy  IEP mRNA:increased expression:brainRGD 
Gabrb1Mousehepatic encephalopathy  ISOGabrb1 (Rattus norvegicus)mRNA:increased expression:brainRGD 
Gabrg2Rathepatic encephalopathy  IEP mRNA:increased expression:brainRGD 
Gabrg2Mousehepatic encephalopathy  ISOGabrg2 (Rattus norvegicus)mRNA:increased expression:brainRGD 

Objects Annotated

Genes (Rattus norvegicus)
Gabra1  (gamma-aminobutyric acid type A receptor subunit alpha 1)
Gabrb1  (gamma-aminobutyric acid type A receptor subunit beta1)
Gabrg2  (gamma-aminobutyric acid type A receptor subunit gamma 2)

Genes (Mus musculus)
Gabra1  (gamma-aminobutyric acid type A receptor subunit alpha 1)
Gabrb1  (gamma-aminobutyric acid type A receptor subunit beta 1)
Gabrg2  (gamma-aminobutyric acid type A receptor, subunit gamma 2)

Genes (Homo sapiens)
GABRA1  (gamma-aminobutyric acid type A receptor subunit alpha1)
GABRB1  (gamma-aminobutyric acid type A receptor subunit beta1)
GABRG2  (gamma-aminobutyric acid type A receptor subunit gamma2)


Additional Information