RGD Reference Report - Deduced primary structure of the beta subunit of brain type II Ca2+/calmodulin-dependent protein kinase determined by molecular cloning. - Rat Genome Database

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Deduced primary structure of the beta subunit of brain type II Ca2+/calmodulin-dependent protein kinase determined by molecular cloning.

Authors: Bennett, MK  Kennedy, MB 
Citation: Bennett MK and Kennedy MB, Proc Natl Acad Sci U S A 1987 Apr;84(7):1794-8.
RGD ID: 634690
Pubmed: PMID:3470758   (View Abstract at PubMed)
PMCID: PMC304527   (View Article at PubMed Central)

cDNA clones coding for the beta subunit of rat brain type II Ca2+/calmodulin-dependent protein kinase were isolated and sequenced. The clones, including one containing the entire coding region, hybridize at high stringency to a single band of poly(A)+ RNA of length 4.8 kilobases. The subunit coded for by the clones was identified by in vitro transcription of the cDNA followed by translation of the resulting RNA. The DNA sequence of the clones contains a single long open reading frame (1626 nucleotides) coding for a protein of 542 amino acids with a molecular weight of 60,333, the amino-terminal half of which is homologous to several other protein kinases. Potential ATP- and calmodulin-binding domains were identified. Two independent clones contain an identical 45-nucleotide deletion, relative to the clones described above, resulting in a shorter open reading frame coding for a protein of molecular weight 58,000. This suggests that the minor, 58-kDa beta' subunit of the type II Ca2+/calmodulin-dependent kinase may be synthesized on a separate message.

Objects referenced in this article
Gene Camk2b calcium/calmodulin-dependent protein kinase II beta Rattus norvegicus

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