RGD Reference Report - Progressive ankylosis (Ank) protein is expressed by neurons and Ank immunohistochemical reactivity is increased by limbic seizures. - Rat Genome Database

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Progressive ankylosis (Ank) protein is expressed by neurons and Ank immunohistochemical reactivity is increased by limbic seizures.

Authors: Yepes, M  Moore, E  Brown, SA  Hanscom, HN  Smith, EP  Lawrence, DA  Winkles, JA 
Citation: Yepes M, etal., Lab Invest 2003 Jul;83(7):1025-32.
RGD ID: 634632
Pubmed: PMID:12861042   (View Abstract at PubMed)

Ank is a 492-amino acid multipass transmembrane protein involved in the regulation of extracellular inorganic pyrophosphate levels and the control of tissue calcification. Previous Northern blot hybridization experiments revealed that Ank mRNA was expressed in the brain, but there have been no reports describing the anatomical sites or specific cell types in the brain that express Ank protein. In this study, we demonstrate that Ank is expressed primarily in human brain neurons, with the highest levels of expression observed in the thalamus, the III and V cortical layers, the Purkinje cells of the cerebellum, clusters of cells in the dorsal portion of the pons and midbrain, and neurons of the anterior horn of the spinal cord. In primary mouse neuronal cell cultures, Ank is detected on both the cell body and on cell extensions, mainly dendrites. In the rat brain, Ank mRNA is expressed at relatively high levels in the thalamus, midbrain, and spinal cord, and the Ank protein expression pattern is similar to that observed in the human brain. Finally, we observed a significant increase in Ank immunoreactivity in the rat amygdala, the CA-2 and CA-3 layers of the hippocampus, and the cerebral cortex after the induction of seizure activity. Ank regulation of ATP and/or inorganic pyrophosphate release from neurons may function to modulate the membrane excitability and cell death associated with seizure activity.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
ANKHHumanExperimental Seizures  ISOAnkh (Rattus norvegicus) RGD 
AnkMouseExperimental Seizures  ISOAnkh (Rattus norvegicus) RGD 
AnkhRatExperimental Seizures  IEP  RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AnkhRatphosphate ion transport  TAS  RGD 
AnkhRatregulation of bone mineralization  TAS  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AnkhRatphosphate transmembrane transporter activity  TAS  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ankh  (ANKH inorganic pyrophosphate transport regulator)

Genes (Mus musculus)
Ank  (progressive ankylosis)

Genes (Homo sapiens)
ANKH  (ANKH inorganic pyrophosphate transport regulator)


Additional Information