RGD Reference Report - AMIGO, a transmembrane protein implicated in axon tract development, defines a novel protein family with leucine-rich repeats. - Rat Genome Database

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AMIGO, a transmembrane protein implicated in axon tract development, defines a novel protein family with leucine-rich repeats.

Authors: Kuja-Panula, J  Kiiltomaki, M  Yamashiro, T  Rouhiainen, A  Rauvala, H 
Citation: Kuja-Panula J, etal., J Cell Biol 2003 Mar 17;160(6):963-73.
RGD ID: 634626
Pubmed: (View Article at PubMed) PMID:12629050
DOI: Full-text: DOI:10.1083/jcb.200209074

Ordered differential display identified a novel sequence induced in neurons by the neurite-promoting protein amphoterin. We named this gene amphoterin-induced gene and ORF (AMIGO), and also cloned two other novel genes homologous to AMIGO (AMIGO2 and AMIGO3). Together, these three AMIGOs form a novel family of genes coding for type I transmembrane proteins which contain a signal sequence for secretion and a transmembrane domain. The deduced extracellular parts of the AMIGOs contain six leucine-rich repeats (LRRs) flanked by cysteine-rich LRR NH2- and COOH-terminal domains and by one immunoglobulin domain close to the transmembrane region. A substrate-bound form of the recombinant AMIGO ectodomain promoted prominent neurite extension in hippocampal neurons, and in solution, the same AMIGO ectodomain inhibited fasciculation of neurites. A homophilic and heterophilic binding mechanism is shown between the members of the AMIGO family. Our results suggest that the members of the AMIGO protein family are novel cell adhesion molecules among which AMIGO is specifically expressed on fiber tracts of neuronal tissues and participates in their formation.

Annotation

Gene Ontology Annotations    

Cellular Component

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Amigo1  (adhesion molecule with Ig like domain 1)
Amigo2  (adhesion molecule with Ig like domain 2)
Amigo3  (adhesion molecule with Ig like domain 3)

Objects referenced in this article
Gene Amigo1 adhesion molecule with Ig like domain 1 Mus musculus
Gene Amigo2 adhesion molecule with Ig like domain 2 Mus musculus
Gene Amigo3 adhesion molecule with Ig like domain 3 Mus musculus

Additional Information